Chromosome Duplication in Saccharomyces cerevisiae

SP Bell, K Labib - Genetics, 2016 - academic.oup.com
The accurate and complete replication of genomic DNA is essential for all life. In eukaryotic
cells, the assembly of the multi-enzyme replisomes that perform replication is divided into …

Rescuing stalled or damaged replication forks

JTP Yeeles, J Poli, KJ Marians… - Cold Spring Harbor …, 2013 - cshperspectives.cshlp.org
In recent years, an increasing number of studies have shown that prokaryotes and
eukaryotes are armed with sophisticated mechanisms to restart stalled or collapsed …

The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

A Yesbolatova, Y Saito, N Kitamoto… - Nature …, 2020 - nature.com
Protein knockdown using the auxin-inducible degron (AID) technology is useful to study
protein function in living cells because it induces rapid depletion, which makes it possible to …

DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in Caenorhabditis elegans

Y Xia, R Sonneville, M Jenkyn-Bedford, L Ji, C Alabert… - Science, 2023 - science.org
Assembly of the CMG (CDC-45–MCM-2-7–GINS) helicase is the key regulated step during
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …

Regulated eukaryotic DNA replication origin firing with purified proteins

JTP Yeeles, TD Deegan, A Janska, A Early, JFX Diffley - Nature, 2015 - nature.com
Eukaryotic cells initiate DNA replication from multiple origins, which must be tightly regulated
to promote precise genome duplication in every cell cycle. To accomplish this, initiation is …

An expanded tool kit for the auxin‐inducible degron system in budding yeast

M Morawska, HD Ulrich - Yeast, 2013 - Wiley Online Library
Fusion of inducible degradation signals, so‐called degrons, to cellular proteins is an elegant
method of controlling protein levels in vivo. Recently, a degron system relying on the plant …

Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation

R Georgescu, Z Yuan, L Bai… - Proceedings of the …, 2017 - National Acad Sciences
The eukaryotic CMG (Cdc45, Mcm2–7, GINS) helicase consists of the Mcm2–7 hexameric
ring along with five accessory factors. The Mcm2–7 heterohexamer, like other hexameric …

MCM: one ring to rule them all

TD Deegan, JFX Diffley - Current opinion in structural biology, 2016 - Elsevier
Highlights•The eukaryotic MCM replicative helicase is tightly regulated during the cell
cycle.•Conformational changes in MCM underpin the different stages of chromosome …

Replication fork activation is enabled by a single-stranded DNA gate in CMG helicase

MR Wasserman, GD Schauer, ME O'Donnell, S Liu - Cell, 2019 - cell.com
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA
at origins yet must transition to single-stranded (ss) DNA for helicase action. CMG must also …

Helicase activation and establishment of replication forks at chromosomal origins of replication

S Tanaka, H Araki - Cold Spring Harbor perspectives in …, 2013 - cshperspectives.cshlp.org
Many replication proteins assemble on the pre-RC-formed replication origins and constitute
the pre-initiation complex (pre-IC). This complex formation facilitates the conversion of Mcm2 …