Numerous PCR-based assays have been developed for the identification of Brucella to
improve diagnostic capabilities. Collectively, the repertoire of assays addresses several …

Brucellosis is a zoonosis caused by facultative intracellular bacteria of the genus Brucella,
which are widely distributed in both humans and animals, especially in the developing …

Real-time PCR-based assays specific for Brucella abortus, Brucella melitensis andBrucella
suis were developed. The assays utilize an upstream primer that is derived from 3′ end of …

Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived
from the virulent strain 2308. The genetic mutations that are responsible for the roughness …

Background Brucellosis affects human populations in many developing countries including
the Middle East, and Latin America where it is still endemic. It has been prevalent in Jordan …

Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification,
which is commonly used to diagnose infectious diseases. The use of PCR for pathogens …

Brucellosis is an ancient disease of animals and man that still threatens the health and
prosperity of many, primarily in the third world, who depend on animal agriculture for their …

One hundred twenty-nine Brucella field strains isolated from cattle in Cantabria, Spain, from
March 1999 to February 2003, were analysed by using the AMOS-ERY PCR assay and by …

The genus Brucella has been described as consisting of six species, three of them including
several biovars, which display a high degree of DNA homology by DNA-DNA hybridization …

La PCR, técnica inventada por Kary B. Mullis en 1983, emplea un par de oligonucleótidos
para delimitar una región de interés y una polimerasa para extenderlos, utilizando ambas …