Designing microbial cell factories for the production of chemicals

JS Cho, GB Kim, H Eun, CW Moon, SY Lee - Jacs Au, 2022 - ACS Publications
The sustainable production of chemicals from renewable, nonedible biomass has emerged
as an essential alternative to address pressing environmental issues arising from our heavy …

Protein engineering for improving and diversifying natural product biosynthesis

C Li, R Zhang, J Wang, LM Wilson, Y Yan - Trends in biotechnology, 2020 - cell.com
Proteins found in nature have traditionally been the most frequently used biocatalysts to
produce numerous natural products ranging from commodity chemicals to pharmaceuticals …

Design of novel enzyme biocatalysts for industrial bioprocess: Harnessing the power of protein engineering, high throughput screening and synthetic biology

A Madhavan, KB Arun, P Binod, R Sirohi… - Bioresource …, 2021 - Elsevier
Biocatalysts have wider applications in various industries. Biocatalysts are generating
bigger attention among researchers due to their unique catalytic properties like activity …

Rational design of enzyme activity and enantioselectivity

Z Song, Q Zhang, W Wu, Z Pu, H Yu - Frontiers in Bioengineering and …, 2023 - frontiersin.org
The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …

Recent progress in the synthesis of advanced biofuel and bioproducts

BF Pfleger, R Takors - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights●Why biofuels and bioproducts remain critical to society●What molecular
products can be made by biotechnology using available feedstocks●How new systems and …

Computational design of enzymes for biotechnological applications

J Planas-Iglesias, SM Marques, GP Pinto, M Musil… - Biotechnology …, 2021 - Elsevier
Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …

From directed evolution to computational enzyme engineering—a review

R Chowdhury, CD Maranas - AIChE Journal, 2020 - Wiley Online Library
Nature relies on a wide range of enzymes with specific biocatalytic roles to carry out much of
the chemistry needed to sustain life. Enzymes catalyze the interconversion of a vast array of …

Microbial synthesis of medium-chain chemicals from renewables

S Sarria, NS Kruyer, P Peralta-Yahya - Nature Biotechnology, 2017 - nature.com
Abstract Linear, medium-chain (C8–C12) hydrocarbons are important components of fuels
as well as commodity and specialty chemicals. As industrial microbes do not contain …

Computational design of highly efficient thermostable MHET hydrolases and dual enzyme system for PET recycling

J Zhang, H Wang, Z Luo, Z Yang, Z Zhang… - Communications …, 2023 - nature.com
Recently developed enzymes for the depolymerization of polyethylene terephthalate (PET)
such as FAST-PETase and LCC-ICCG are inhibited by the intermediate PET product mono …

Fatty acid biosynthesis: chain‐length regulation and control

CS Heil, SS Wehrheim, KS Paithankar… - …, 2019 - Wiley Online Library
De novo biosynthesis of fatty acids is an iterative process requiring strict regulation of the
lengths of the produced fatty acids. In this review, we focus on the factors determining chain …