The extracellular matrix is a critical component of microbial biofilms, such as dental plaque,
maintaining the spatial arrangement of cells and coordinating cellular functions throughout …

A variety of organisms, such as bacteria, fungi, and plants, produce secondary metabolites,
also known as natural products. Natural products have been a prolific source and an …

One essential prokaryotic cell function is the transport of proteins from the cytoplasm into
other compartments of the cell, the environment, and/or other bacteria or eukaryotic cells—a …

The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into
the culture supernatant of a bacterial expression host greatly facilitates their downstream …

The signal recognition particle (SRP) and its receptor compose a universally conserved and
essential cellular machinery that couples the synthesis of nascent proteins to their proper …

Highlights•The need for recombinant proteins both as biopharmaceuticals and for structural
studies in drug development is outlined.•The basis of the production of recombinant proteins …

Almost all of the 200 or so approved biopharmaceuticals have been produced in one of
three host systems: the bacterium Escherichia coli, yeasts (Saccharomyces cerevisiae …

Autotransport in Gram-negative bacteria denotes the ability of surface-localized proteins to
cross the outer membrane (OM) autonomously. Autotransporters perform this task with the …

Short (15–30 residue) chains of amino acids at the amino termini of expressed proteins
known as signal peptides (SPs) specify secretion in living cells. We trained an attention …

There is a lack of new antibiotics to combat drug-resistant bacterial infections that
increasingly threaten global health. The current pipeline of clinical-stage antimicrobials is …