Small‐Molecule Quenchers for Förster Resonance Energy Transfer: Structure, Mechanism, and Applications

B Fang, Y Shen, B Peng, H Bai, L Wang… - Angewandte …, 2022 - Wiley Online Library
For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely
used mechanisms to realize the detection of biochemical activities. Dark quenchers relax …

DNA-mediated homogeneous binding assays for nucleic acids and proteins

H Zhang, F Li, B Dever, XF Li, XC Le - Chemical reviews, 2013 - ACS Publications
The detection of nucleic acids and proteins is fundamental for studying their functions and
for the development of molecular diagnostics. These molecules carry out most biological …

Fatty acid carbon is essential for dNTP synthesis in endothelial cells

S Schoors, U Bruning, R Missiaen, KCS Queiroz… - Nature, 2015 - nature.com
The metabolism of endothelial cells during vessel sprouting remains poorly studied. Here
we report that endothelial loss of CPT1A, a rate-limiting enzyme of fatty acid oxidation (FAO) …

HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase

DC Goldstone, V Ennis-Adeniran, JJ Hedden… - Nature, 2011 - nature.com
SAMHD1, an analogue of the murine interferon (IFN)-γ-induced gene Mg11 (ref.), has
recently been identified as a human immunodeficiency virus-1 (HIV-1) restriction factor that …

Suppression of nucleotide metabolism underlies the establishment and maintenance of oncogene-induced senescence

KM Aird, G Zhang, H Li, Z Tu, BG Bitler, A Garipov… - Cell reports, 2013 - cell.com
Oncogene-induced senescence is characterized by a stable cell growth arrest, thus
providing a tumor suppression mechanism. However, the underlying mechanisms for this …

Adaptive Reprogramming of De Novo Pyrimidine Synthesis Is a Metabolic Vulnerability in Triple-Negative Breast Cancer

KK Brown, JB Spinelli, JM Asara, A Toker - Cancer discovery, 2017 - AACR
Chemotherapy resistance is a major barrier to the treatment of triple-negative breast cancer
(TNBC), and strategies to circumvent resistance are required. Using in vitro and in vivo …

NUDT15 hydrolyzes 6-thio-deoxyGTP to mediate the anticancer efficacy of 6-thioguanine

NCK Valerie, A Hagenkort, BDG Page, G Masuyer… - Cancer research, 2016 - AACR
Thiopurines are a standard treatment for childhood leukemia, but like all chemotherapeutics,
their use is limited by inherent or acquired resistance in patients. Recently, the nucleoside …

SAMHD1‐dependent retroviral control and escape in mice

J Rehwinkel, J Maelfait, A Bridgeman, R Rigby… - The EMBO …, 2013 - embopress.org
SAMHD1 is a host restriction factor for human immunodeficiency virus 1 (HIV‐1) in cultured
human cells. SAMHD1 mutations cause autoimmune Aicardi‐Goutières syndrome and are …

ATM couples replication stress and metabolic reprogramming during cellular senescence

KM Aird, AJ Worth, NW Snyder, JV Lee, S Sivanand… - Cell reports, 2015 - cell.com
Replication stress induced by nucleotide deficiency plays an important role in cancer
initiation. Replication stress in primary cells typically activates the cellular senescence tumor …

SAMHD1 is down regulated in lung cancer by methylation and inhibits tumor cell proliferation

J Wang, F Lu, XY Shen, Y Wu, L Zhao - Biochemical and biophysical …, 2014 - Elsevier
The function of dNTP hydrolase SAMHD1 as a viral restriction factor to inhibit the replication
of several viruses in human immune cells was well established. However, its regulation and …