In vitro display technologies, best exemplified by phage and yeast display, were first
described for the selection of antibodies some 20 years ago. Since then, many antibodies …

Antibodies constitute the most rapidly growing class of human therapeutics and the second
largest class of drugs after vaccines. The generation of potent antibody therapeutics, which I …

This protocol describes the process of isolating and engineering antibodies or proteins for
increased affinity and stability using yeast surface display. Single-chain antibody fragments …

We have used homologous recombination in ES cells to engineer B cell-deficient mice that
are incapable of expressing endogenous immunoglobulin heavy and kappa light chain …

Yeast surface display is being employed to engineer desirable properties into proteins for a
broad variety of applications. Labeling with soluble ligands enables rapid and quantitative …

Currently, the reliable detection and quantification of a multitude of different analytes is
crucial in many applications and settings. Biosensors have revolutionised diagnostics for …

Intrinsically disordered proteins (IDPs) are recognized to play important roles in many
biological functions such as transcription and translation regulation, cellular signal …

Affinity reagents are essential tools in both basic and applied research; however, there is a
growing concern about the reproducibility of animal-derived monoclonal antibodies. The …

Yeast surface display has become an increasingly popular tool for protein engineering and
library screening applications. Recent advances have greatly expanded the capability of …

The majority of broadly neutralizing antibodies to hepatitis C virus (HCV) are against
conformational epitopes on the E2 glycoprotein. Many of them recognize overlapping …