Discovered as organometallic curiosities in the 1970s, carbene radicals have become a
staple in modern-day homogeneous catalysis. Carbene radicals exhibit nucleophilic radical …

The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …

Cytochrome P450 enzymes (P450s) catalyze diverse oxidative cross-coupling reactions
between aromatic substrates in the natural product biosynthesis. Specifically, P450s install …

The conversion of inexpensive aqueous ammonia (NH3· H2O) into value-added primary
amines by N− H insertion persists as a longstanding challenge in chemistry because of the …

Free carbene readily causes multiple side reactions due to its high energy, thus its
asymmetric transformation is very difficult. We present here our findings of high‐pKa …

We report enantioselective one-carbon ring expansion of aziridines to make azetidines as a
new-to-nature activity of engineered “carbene transferase” enzymes. A laboratory-evolved …

Transition-metal-catalyzed carbene insertion reactions of a nitrogen–hydrogen bond have
emerged as robust and versatile methods for the construction of C–N bonds. While …

New-to-nature radical biocatalysis has recently emerged as a powerful strategy to tame
fleeting open-shell intermediates for stereoselective transformations. In 2021, we introduced …

Transition-metal-catalyzed enantioselective N–H insertion reactions of carbene species offer
a powerful and straightforward strategy to produce chiral nitrogen-containing compounds …

In the context of carbon peaking and carbon neutrality, the recovery and utilization of clean
CO2 in energy storage systems have attracted significant attention from researchers …