Interactions between proteins are orchestrated in a precise and time-dependent manner,
underlying cellular function. The binding affinity, defined as the strength of these …

The influence of a xenobiotic compound on an organism is usually summarized by the
expression biological activity. If a controlled, therapeutically relevant, and regulatory action …

We have developed a computer algorithm, FOLDEF (for FOLD-X energy function), to provide
a fast and quantitative estimation of the importance of the interactions contributing to the …

Denaturant m values, the dependence of the free energy of unfolding on denaturant
concentration, have been collected for a large set of proteins. The m value correlates very …

Soluble luminescent temperature probes are promising candidates for optical thermometry
and thermography applications requiring precise, passive, and spatially resolved …

The principles of isothermal titration calorimetry (ITC) and differential scanning calorimetry
(DSC) are reviewed together with the basic thermodynamic formalism on which the two …

The interaction of biologicalmacromolecules, whether protein–DNA, antibody–antigen,
hormone–receptor, etc., illustrates the complexity and diversity of molecular recognition. The …

The tendency of proteins to spontaneously adopt a well-defined conformation in solution has
intrigued investigators for many decades. 1 The key questions in the study of this …

Publisher Summary This chapter summarizes the experimental information on protein
energetics. This field is developing fast and the concept of the energetics of protein structure …

The interactions between a series of oppositely charged polypeptide pairs are probed using
isothermal titration calorimetry (ITC) in combination with turbidity measurements and optical …