The compartmentalization of eukaryotic cells is reliant on the fidelity of vesicle-mediated
intracellular transport. Vesicles deliver their cargo via membrane fusion, a process requiring …

The conserved oligomeric Golgi (COG) complex, a multisubunit tethering complex of the
CATCHR (complexes associated with tethering containing helical rods) family, controls …

Lysosomes are essential for cellular recycling, nutrient signaling, autophagy, and
pathogenic bacteria and viruses invasion. Lysosomal fusion is fundamental to cell survival …

Negative stain electron microscopy (EM) allows relatively simple and quick observation of
macromolecules and macromolecular complexes through the use of contrast enhancing …

The essential Golgi protein Sly1 is a member of the Sec1/mammalian Unc-18 (SM) family of
SNARE chaperones. Sly1 was originally identified through remarkable gain-of-function …

Vesicular transport between different membrane compartments is a key process in cell
biology required for the exchange of material and information. The complex machinery that …

The structural characterization of protein complexes in their native environment is
challenging but crucial for understanding the mechanisms that mediate cellular processes …

A major challenge for a molecular understanding of membrane trafficking has been the
elucidation of high-resolution structures of large, multisubunit tethering complexes that …

Highlights•Coiled-coil golgin proteins capture transport vesicles in a specific
manner.•Golgins contain vesicle tethering motifs.•Multi-subunit tethering complexes (MTCs) …

Abstract The Conserved Oligomeric Golgi (COG) complex, a multi-subunit vesicle tethering
complex of the CATCHR (Complexes Associated with Tethering Containing Helical Rods) …