Receptor tyrosine kinase-mediated growth factor signaling is essential for proper formation
and development of the neural crest. The many ligands and receptors implicated in these …

Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in
developmental, cell, and molecular biology. These dual frog species boast experimental …

Animal models of X-linked juvenile retinoschisis (XLRS) are valuable tools for
understanding basic biochemical function of retinoschisin (RS1) protein and to investigate …

Precise single‐base editing mXenopus tropicalis would greatly expand the utility of this true
diploid frog for modeling human genetic diseases caused by point mutations. Here, we …

The targeted nuclease revolution (TALENs, CRISPR/Cas9) now allows Xenopus
researchers to rapidly generate custom on‐demand genetic knockout models. These novel …

The focal adhesion protein Kindlin2 is essential for integrin activation, a process that is
fundamental to cell-extracellular matrix adhesion. Kindlin 2 (Fermt2) is widely expressed in …

Analysis of large-scale human genomic data has yielded unexplained mutations known to
cause severe disease in healthy individuals. Here, we report the unexpected recovery of a …

Myogenic regulatory factors (MRFs) are known to have essential roles in both the
establishment and differentiation of the skeletal muscle cell lineage. MyoD is expressed …

In this chapter, we convey a state-of-the art update to the 2014 Nakayama protocol for
CRISPR/Cas9 genome engineering in Xenopus tropicalis (X. tropicalis). We discuss in …

Animal models are useful to study the molecular, cellular, and morphogenetic mechanisms
underlying normal and pathological development. Cell‐based study models have emerged …