Proteomics of human biological fluids for biomarker discoveries: technical advances and recent applications

L Dayon, O Cominetti, M Affolter - Expert review of proteomics, 2022 - Taylor & Francis
Introduction Biological fluids are routine samples for diagnostic testing and monitoring.
Blood samples are typically measured because of their moderate invasive collection and …

Identification of tear-based protein and non-protein biomarkers: Its application in diagnosis of human diseases using biosensors

SK Nandi, D Singh, J Upadhay, N Gupta… - International Journal of …, 2021 - Elsevier
Discovery of robust, selective and specific biomarkers are important for early diagnosis and
monitor progression of human diseases. Eye being a common target for several human …

Comparison of different mass spectrometry workflows for the proteomic analysis of tear fluid

G Jones, TJ Lee, J Glass, G Rountree, L Ulrich… - International journal of …, 2022 - mdpi.com
The tear film is a multi-layer fluid that covers the corneal and conjunctival epithelia of the eye
and provides lubrication, nutrients, and protection from the outside environment. Tear fluid …

Proteomic analysis of tears and conjunctival cells collected with Schirmer strips using timsTOF Pro: Preanalytical considerations

M Akkurt Arslan, I Kolman, C Pionneau, S Chardonnet… - Metabolites, 2021 - mdpi.com
This study aimed to investigate the human proteome profile of samples collected from whole
(W) Schirmer strips (ScS) and their two parts—the bulb (B) and the rest of the strip (R)—with …

Targeted Workflow Investigating Variations in the Tear Proteome by Liquid Chromatography Tandem Mass Spectrometry

M Lépine, O Zambito, L Sleno - ACS omega, 2023 - ACS Publications
Proteins in tears have an important role in eye health and have been shown as a promising
source of disease biomarkers. The goal of this study was to develop a robust, sensitive, and …

Clinical tear fluid proteomics—a novel tool in glaucoma research

J Nättinen, U Aapola, P Nukareddy… - International Journal of …, 2022 - mdpi.com
Tear fluid forms the outermost layer of the ocular surface and its characteristics and
composition have been connected to various ocular surface diseases. As tear proteomics …

[HTML][HTML] Tear proteomics in dry eye disease

R Kannan, S Das, R Shetty, L Zhou… - Indian Journal of …, 2023 - journals.lww.com
Dry eye disease (DED) is a multi-factorial ocular surface condition driven by compromised
ocular lubrication and inflammation which leads to itching, dryness, and vision impairment …

The amniotic fluid proteome changes across gestation in humans and rhesus macaques

LE Shorey-Kendrick, BA Crosland, ER Spindel… - Scientific Reports, 2023 - nature.com
Amniotic fluid is a complex biological medium that offers protection to the fetus and plays a
key role in normal fetal nutrition, organogenesis, and potentially fetal programming. Amniotic …

A Look into Ocular Diseases: The Pivotal Role of Omics Sciences in Ophthalmology Research

M Fucito, M Spedicato, S Felletti, AC Yu… - ACS Measurement …, 2024 - ACS Publications
Precision medicine is a new medical approach which considers both population
characteristics and individual variability to provide customized healthcare. The transition …

Antibody and protein profiles in glaucoma: screening of biomarkers and identification of signaling pathways

N Auler, H Tonner, N Pfeiffer, FH Grus - Biology, 2021 - mdpi.com
Simple Summary Glaucoma is a chronic eye disease that is one of the leading causes of
blindness worldwide. Currently, the only therapeutic option is to lower intraocular pressure …