Applications of nanobodies
S Muyldermans - Annual review of animal biosciences, 2021 - annualreviews.org
Unique, functional, homodimeric heavy chain–only antibodies, devoid of light chains, are
circulating in the blood of Camelidae. These antibodies recognize their cognate antigen via …
circulating in the blood of Camelidae. These antibodies recognize their cognate antigen via …
[HTML][HTML] Super-resolution microscopy for structural cell biology
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …
A guide to: generation and design of nanobodies
S Muyldermans - The FEBS journal, 2021 - Wiley Online Library
A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding
domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are …
domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are …
Switchable fluorophores for single-molecule localization microscopy
H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …
microscopy methods in biology and other fields. Single-molecule localization microscopy …
Nanobody: outstanding features for diagnostic and therapeutic applications
JP Salvador, L Vilaplana, MP Marco - Analytical and bioanalytical …, 2019 - Springer
Nanobodies (Nbs) have arisen as an alternative to conventional antibodies (Abs) and show
great potential when used as tools in different biotechnology fields such as diagnostics and …
great potential when used as tools in different biotechnology fields such as diagnostics and …
[HTML][HTML] A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging
D Virant, B Traenkle, J Maier, PD Kaiser… - Nature …, 2018 - nature.com
Dense fluorophore labeling without compromising the biological target is crucial for genuine
super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for …
super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for …
[HTML][HTML] A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies
Polyclonal anti–immunoglobulin G (anti-IgG) secondary antibodies are essential tools for
many molecular biology techniques and diagnostic tests. Their animal-based production is …
many molecular biology techniques and diagnostic tests. Their animal-based production is …
Recombinant expression of nanobodies and nanobody-derived immunoreagents
A De Marco - Protein expression and purification, 2020 - Elsevier
Antibody fragments for which the sequence is available are suitable for straightforward
engineering and expression in both eukaryotic and prokaryotic systems. When produced as …
engineering and expression in both eukaryotic and prokaryotic systems. When produced as …
[HTML][HTML] Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies
OS Oliinyk, M Baloban, CL Clark, E Carey, S Pletnev… - Nature …, 2022 - nature.com
Small near-infrared (NIR) fluorescent proteins (FPs) are much needed as protein tags for
imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which …
imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which …
[HTML][HTML] Bioorthogonal labeling with tetrazine-dyes for super-resolution microscopy
G Beliu, AJ Kurz, AC Kuhlemann… - Communications …, 2019 - nature.com
Genetic code expansion (GCE) technology allows the specific incorporation of
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …