Unique, functional, homodimeric heavy chain–only antibodies, devoid of light chains, are
circulating in the blood of Camelidae. These antibodies recognize their cognate antigen via …

Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding
domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are …

The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Nanobodies (Nbs) have arisen as an alternative to conventional antibodies (Abs) and show
great potential when used as tools in different biotechnology fields such as diagnostics and …

Dense fluorophore labeling without compromising the biological target is crucial for genuine
super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for …

Polyclonal anti–immunoglobulin G (anti-IgG) secondary antibodies are essential tools for
many molecular biology techniques and diagnostic tests. Their animal-based production is …

Antibody fragments for which the sequence is available are suitable for straightforward
engineering and expression in both eukaryotic and prokaryotic systems. When produced as …

Small near-infrared (NIR) fluorescent proteins (FPs) are much needed as protein tags for
imaging applications. We developed a 17 kDa NIR FP, called miRFP670nano3, which …

Genetic code expansion (GCE) technology allows the specific incorporation of
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …