Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Fluorescence lifetime imaging provides more possibility of in vivo multiplexing in second
near infrared (NIR‐II) window. However, it still faces the obstacle that fluorescent probes with …

In this study, we integrate a single-photon detector array into a confocal laser scanning
microscope, enabling the combination of fluorescence-lifetime single-molecule localization …

Localization of single fluorescent emitters is key for physicochemical and biophysical
measurements at the nanoscale and beyond ensemble averaging. Examples include single …

In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Phasor plots and the future of spectral and lifetime imaging | Nature Methods Skip to main
content Thank you for visiting nature.com. You are using a browser version with limited support …

Super-resolution optical imaging is crucial to the study of cellular processes. Current super-
resolution fluorescence microscopy is restricted by the need of special fluorophores or …

We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-
molecule localization method with a simplified experimental setup and additional …

Recent advances in technology have allowed the production and the coherent detection of
sub-ps pulses of terahertz (THz) radiation. Therefore, the potentialities of this technique have …