Renaissance of protein crystallization and precipitation in biopharmaceuticals purification

R Dos Santos, AL Carvalho, ACA Roque - Biotechnology advances, 2017 - Elsevier
The current chromatographic approaches used in protein purification are not keeping pace
with the increasing biopharmaceutical market demand. With the upstream improvements …

Structure of a paramyxovirus polymerase complex reveals a unique methyltransferase-CTD conformation

R Abdella, M Aggarwal, T Okura… - Proceedings of the …, 2020 - National Acad Sciences
Paramyxoviruses are enveloped, nonsegmented, negative-strand RNA viruses that cause a
wide spectrum of human and animal diseases. The viral genome, packaged by the …

Recombinant soluble respiratory syncytial virus F protein that lacks heptad repeat B, contains a GCN4 trimerization motif and is not cleaved displays prefusion-like …

I Widjaja, A Rigter, S Jacobino, FJM van Kuppeveld… - PloS one, 2015 - journals.plos.org
The respiratory syncytial virus (RSV) fusion protein F is considered an attractive vaccine
candidate especially in its prefusion conformation. We studied whether recombinant soluble …

Flexibility of the head-stalk linker domain of paramyxovirus HN glycoprotein is essential for triggering virus fusion

E Adu-Gyamfi, LS Kim, TS Jardetzky… - Journal of virology, 2016 - Am Soc Microbiol
The Paramyxoviridae comprise a large family of enveloped, negative-sense, single-stranded
RNA viruses with significant economic and public health implications. For nearly all …

Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant

BJ Feehan, AA Penin, AN Mukhin, D Kumar… - Viruses, 2019 - mdpi.com
A distinct Russian Mammalian orthorubulavirus 5 (PIV5) was detected in cell culture
exhibiting cytopathic effect and hypothesized to be contaminated by a scientist with …

Immobilization of the N-terminal helix stabilizes prefusion paramyxovirus fusion proteins

AS Song, TA Poor, LA Abriata… - Proceedings of the …, 2016 - National Acad Sciences
Parainfluenza virus 5 (PIV5) is an enveloped, single-stranded, negative-sense RNA virus of
the Paramyxoviridae family. PIV5 fusion and entry are mediated by the coordinated action of …

Cryogenic-Electron Microscopy Structures of Viral and Eukaryotic Transcription Complexes

R Abdella - 2021 - search.proquest.com
Transcription of nucleic acids (DNA and RNA) is a hallmark of life, taking the information
stored within genomic nucleic acids and converting it into a form that is useful for producing …

Developing non-chromatographic strategies for biopharmaceuticals purification

RAR dos Santos - 2020 - search.proquest.com
The biopharmaceutical market shows a high annual growth, and the major contributors for
this success are antibody molecules. The purification of high added value biological …

Interferon induction by paramyxoviruses: investigations into specific RNA: protein interactions

F Dominguez Palao - 2017 - research-repository.st-andrews.ac …
RNA: protein interactions are central in many cellular processes, including activation of
innate immune responses against microbial infection. Their study is essential to better …

[引用][C] 新城疫病毒包膜糖蛋白的结构与功能

刘亚轻, 王志玉 - 病毒学报, 2018