[HTML][HTML] Label-free SARS-CoV-2 detection and classification using phase imaging with computational specificity

N Goswami, YR He, YH Deng, C Oh, N Sobh… - Light: Science & …, 2021 - nature.com
Efforts to mitigate the COVID-19 crisis revealed that fast, accurate, and scalable testing is
crucial for curbing the current impact and that of future pandemics. We propose an optical …

[HTML][HTML] Epi-illumination gradient light interference microscopy for imaging opaque structures

ME Kandel, C Hu, G Naseri Kouzehgarani… - Nature …, 2019 - nature.com
Multiple scattering and absorption limit the depth at which biological tissues can be imaged
with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field …

Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure

ME Kandel, M Rubessa, YR He… - Proceedings of the …, 2020 - National Acad Sciences
The ability to evaluate sperm at the microscopic level, at high-throughput, would be useful
for assisted reproductive technologies (ARTs), as it can allow specific selection of sperm …

Limited-angle tomographic phase microscopy utilizing confocal scanning fluorescence microscopy

R Guo, I Barnea, NT Shaked - Biomedical Optics Express, 2021 - opg.optica.org
We present a multimodal imaging technique, combining tomographic phase microscopy with
limited angular projection range and number, and two-channel spinning-disk confocal …

High-resolution 4-D acquisition of freely swimming human sperm cells without staining

G Dardikman-Yoffe, SK Mirsky, I Barnea… - Science advances, 2020 - science.org
We present a new acquisition method that enables high-resolution, fine-detail full
reconstruction of the three-dimensional movement and structure of individual human sperm …

Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

M Rubessa, MB Wheeler - Theriogenology, 2020 - Elsevier
In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various
species. However, there is no consensus or gold standard method on how to assess their …

Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos

MB Wheeler, RAC Rabel, M Rubessa… - Biology of …, 2024 - academic.oup.com
Assisted reproduction is one of the significant tools to treat human infertility. Morphological
assessment is the primary method to determine sperm and embryo viability during IVF …

SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle

M Rubessa, SN Lotti, ME Kandel, G Popescu… - Molecular biology …, 2019 - Springer
Naked DNA has been shown to bind naturally to the sperm, a method called sperm-
mediated gene transfer (SMGT). Based on these observations, we examined the efficiency …

Quantitative label-free technique for morphological evaluation of human sperm—a promising tool in semen evaluation

PK Poola, V Jayaraman, K Chaithanya, D Rao… - OSA …, 2018 - opg.optica.org
We present results from non-interferometric quantitative phase imaging (QPI) for thickness
estimation and morphological analysis of human spermatozoa. QPI along with algorithms for …

Cell and nucleus refractive‐index mapping by interferometric phase microscopy and rapid confocal fluorescence microscopy

S Cohen‐Maslaton, I Barnea, A Taieb… - Journal of …, 2020 - Wiley Online Library
We present a multimodal technique for measuring the integral refractive index and the
thickness of biological cells and their organelles by integrating interferometric phase …