This article reviews technology trends in antibody purification. Section 1 discusses non-
chromatography methods, including precipitation, liquid–liquid extraction, and high …

The global antibody market has grown exponentially due to increasing applications in
research, diagnostics and therapy. Antibodies are present in complex matrices (eg serum …

Following the consolidation of therapeutic proteins in the fight against cancer, autoimmune,
and neurodegenerative diseases, recent advancements in biochemistry and biotechnology …

Electrospun hybrid chitosan/nylon-6 nanofibrous mats with fiber diameters in the range of 80–
310 nm were successfully fabricated using an electrospinning method. Nanofibrous …

Protein A (SpA) affinity chromatography has been widely used for the purification of
immunoglobulin G (IgG). However, the molecular mechanism of the affinity between IgG and …

Nonwovens fabrics are porous materials with great potential for use in the separation and
purification of biotherapeutic proteins and other biomolecules after proper surface activation …

Hydrophobic charge-induction chromatography (HCIC) with 4-mercaptoethyl-pyridine (MEP)
as the ligand is a novel technology for antibody purification. In the present work, the …

Molecularly imprinted polymers (MIP) offer in principle a robust, cost-efficient alternative to
antibodies, but it is still a challenge to develop such materials for protein recognition. Here …

The rapid increase in antibody demand is urgently calling for high throughput technologies
to enhance the efficiency in downstream purification. Membrane chromatography using …

Protein A from the bacterium Staphylococcus aureus (SpA) has been widely used as an
affinity ligand for purification of immunoglobulin G (IgG). The affinity between SpA and IgG is …