The advent of genome editing techniques based on the clustered regularly interspersed
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …

Argonaute (AGO) proteins associate with small RNAs to direct their effector function on
complementary transcripts. The nematode Caenorhabditis elegans contains an expanded …

Experimental manipulation of protein abundance in living cells or organisms is an essential
strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques …

Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease
Cas9 has become a popular method for genome editing in several organisms. Most HDR …

It is not known at present whether neuronal cell-type diversity—defined by cell-type-specific
anatomical, biophysical, functional and molecular signatures—can be reduced to relatively …

The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …

The formation of synapses during neuronal development is essential for establishing neural
circuits and a nervous system. Every presynapse builds a core 'active zone'structure, where …

The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

Autophagy is a cellular degradation process important for neuronal development and
survival. Neurons are highly polarized cells in which autophagosome biogenesis is spatially …

Mobile genetic elements threaten genome integrity in all organisms. RDE-3 (also known as
MUT-2) is a ribonucleotidyltransferase that is required for transposon silencing and RNA …