Metabolic Engineering of Escherichia coli for High-Level Production of Lacto-N-neotetraose and Lacto-N-tetraose

Y Liao, J Wu, Z Li, J Wang, L Yuan, C Lao… - Journal of Agricultural …, 2023 - ACS Publications
Lacto-N-neotetraose (LNnT) and lacto-N-tetraose (LNT) are important oligosaccharides
found in breast milk and are commonly used as nutritional supplements in infant formula. We …

Improving microbial bioproduction under low-oxygen conditions

S Kulakowski, D Banerjee, CD Scown… - Current Opinion in …, 2023 - Elsevier
Highlights•Low-oxygen production can expand the range of industrially biomanufactured
chemicals.•Rewiring cofactor utilization in metabolism can reduce cellular oxygen …

Bioprocessing of inclusion bodies from E. coli. to produce bioactive recombinant proteins

AK Rani, R Katiyar, AS Rathore - Biochemical Engineering Journal, 2024 - Elsevier
Recombinant protein production has revolutionized healthcare with biopharmaceutical
products, dominating the pharmaceutical pipelines today. E. coli remains one of the most …

Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling

S Abdelrazig, L Safo, GA Rance, MW Fay… - RSC …, 2020 - pubs.rsc.org
Magnetosomes are nano-sized magnetic nanoparticles with exquisite properties that can be
used in a wide range of healthcare and biotechnological applications. They are …

Recombinant protein expression in proteome‐reduced cells under aerobic and oxygen‐limited regimes

AR Lara, J Utrilla, LM Martínez… - Biotechnology and …, 2024 - Wiley Online Library
Industrial cultures are hindered by the physiological complexity of the host and the limited
mass transfer capacity of conventional bioreactors. In this study, a minimal cell approach …

Microaerobic Fermentation Enables High-Titer Biosynthesis of the Rose Monoterpenes Geraniol and Geranyl Acetate in Escherichia coli

S Shukal, L Ong, X Chen, C Zhang - ACS Sustainable Chemistry …, 2024 - ACS Publications
Monoterpenes are commercially important flavors and fragrances with high demand.
Microbial production of monoterpenes is more sustainable than plant extraction, however, it …

Optimized Enzyme Production for the Escherichia coli Whole-Cell Biocatalytic Synthesis of Codeine from Thebaine

A Jahanian, A Velasquez Agudelo… - … Process Research & …, 2024 - ACS Publications
Codeine, the most widely used medicinal opiate in the world, can be produced from
thebaine by using an environmentally friendly biocatalytic process using purified enzymes or …

The interplay of active energy harvesting and wastewater organic loading regulates fermentation products and microbiomes in microbial fuel cells

X Mei, B Lu, C Yan, J Gu, N Ren, ZJ Ren… - Resources, Conservation …, 2022 - Elsevier
Fruit juice wastewater (FJW) contains high strength and acidic organics that are difficult to
remove. We developed microbial fuel cells (MFCs) to treat FJW and employed new energy …

On-line analysis of bacterial metabolism by modern spectroscopic laser techniques

GD Metcalfe - 2022 - etheses.whiterose.ac.uk
In order to best determine how bioprocesses develop and how to run bioreactors most
efficiently, innovative new analytical techniques are required to supplement, or even …

Lacto-N-Neotetraose

M Zhang, T Liu, S Chen, K Zhang - Biomanufacture of Functional … - taylorfrancis.com
Human milk oligosaccharides (HMOs) are a class of biologically active components
abundant in breast milk, which have drawn significant attention because of their essential …