The green fluorescent protein

RY Tsien - Annual review of biochemistry, 1998 - annualreviews.org
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria
has vaulted from obscurity to become one of the most widely studied and exploited proteins …

Understanding, improving and using green fluorescent proteins

AB Cubitt, R Heim, SR Adams, AE Boyd… - Trends in biochemical …, 1995 - cell.com
Green fluorescent proteins (GFPs) are presently attracting tremendous interest as the first
general method to create strong visible fluorescence by purely molecular biological means …

The molecular structure of green fluorescent protein

F Yang, LG Moss, GN Phillips Jr - Nature biotechnology, 1996 - nature.com
The crystal structure of recombinant wild-type green fluorescent protein (GFP) has been
solved to a resolution of 1.9 Å by multiwavelength anomalous dispersion phasing methods …

Generation of destabilized green fluorescent protein as a transcription reporter

X Li, X Zhao, Y Fang, X Jiang, T Duong, C Fan… - Journal of Biological …, 1998 - ASBMB
The green fluorescent protein (GFP) is a widely used reporter in gene expression and
protein localization studies. GFP is a stable protein; this property allows its accumulation and …

Recombinant probes for visualizing endogenous synaptic proteins in living neurons

GG Gross, JA Junge, RJ Mora, HB Kwon, CA Olson… - Neuron, 2013 - cell.com
The ability to visualize endogenous proteins in living neurons provides a powerful means to
interrogate neuronal structure and function. Here we generate recombinant antibody-like …

Methods for marking insects: current techniques and future prospects

JR Hagler, CG Jackson - Annual review of entomology, 2001 - annualreviews.org
▪ Abstract Tracking the movement of insects in their natural habitat is essential for
understanding their basic biology, demography, and ethology. A wide variety of markers …

Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase

TG Banke, D Bowie, HK Lee, RL Huganir… - Journal of …, 2000 - Soc Neuroscience
Modulation of postsynaptic AMPA receptors in the brain by phosphorylation may play a role
in the expression of synaptic plasticity at central excitatory synapses. It is known from …

[HTML][HTML] A family of sulfonylurea receptors determines the pharmacological properties of ATP-sensitive K+ channels

N Inagaki, T Gonoi, JPC Iv, CZ Wang, L Aguilar-Bryan… - Neuron, 1996 - cell.com
We have cloned an isoform of the sulfonylurea receptor (SUR), designated SUR2.
Coexpression of SUR2 and the inward rectifier K+ channel subunit Kir6. 2 in COS1 cells …

A genetically encoded optical probe of membrane voltage

MS Siegel, EY Isacoff - Neuron, 1997 - cell.com
Measuring electrical activity in large numbers of cells with high spatial and temporal
resolution is a fundamental problem for the study of neural development and information …

NMDA receptor-dependent synaptic reinforcement as a crucial process for memory consolidation

E Shimizu, YP Tang, C Rampon, JZ Tsien - Science, 2000 - science.org
The hippocampal CA1 region is crucial for converting new memories into long-term
memories, a process believed to continue for week (s) after initial learning. By developing an …