[HTML][HTML] Cannabis sativa: The Plant of the Thousand and One Molecules

CM Andre, JF Hausman, G Guerriero - Frontiers in plant science, 2016 - frontiersin.org
Cannabis sativa L. is an important herbaceous species originating from Central Asia, which
has been used in folk medicine and as a source of textile fiber since the dawn of times. This …

Advancing crop transformation in the era of genome editing

F Altpeter, NM Springer, LE Bartley, AE Blechl… - The Plant …, 2016 - academic.oup.com
Plant transformation has enabled fundamental insights into plant biology and revolutionized
commercial agriculture. Unfortunately, for most crops, transformation and regeneration …

Engineering bacteria for diagnostic and therapeutic applications

DT Riglar, PA Silver - Nature Reviews Microbiology, 2018 - nature.com
Our ability to generate bacterial strains with unique and increasingly complex functions has
rapidly expanded in recent times. The capacity for DNA synthesis is increasing and costing …

A highly characterized yeast toolkit for modular, multipart assembly

ME Lee, WC DeLoache, B Cervantes… - ACS synthetic …, 2015 - ACS Publications
Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

SlWRKY35 positively regulates carotenoid biosynthesis by activating the MEP pathway in tomato fruit

Y Yuan, S Ren, X Liu, L Su, Y Wu, W Zhang… - New …, 2022 - Wiley Online Library
Carotenoids are vital phytonutrients widely recognised for their health benefits. Therefore, it
is vital to thoroughly investigate the metabolic regulatory network underlying carotenoid …

A golden gate modular cloning toolbox for plants

C Engler, M Youles, R Gruetzner, TM Ehnert… - ACS synthetic …, 2014 - ACS Publications
Plant Synthetic Biology requires robust and efficient methods for assembling multigene
constructs. Golden Gate cloning provides a precision module-based cloning technique for …

A user's guide to golden gate cloning methods and standards

JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology

A Sarrion-Perdigones, M Vazquez-Vilar… - Plant …, 2013 - academic.oup.com
Plant synthetic biology aims to apply engineering principles to plant genetic design. One
strategic requirement of plant synthetic biology is the adoption of common standardized …

Standards for plant synthetic biology: a common syntax for exchange of DNA parts

NJ Patron, D Orzaez, S Marillonnet… - New …, 2015 - Wiley Online Library
Inventors in the field of mechanical and electronic engineering can access multitudes of
components and, thanks to standardization, parts from different manufacturers can be used …

Bricks and blueprints: methods and standards for DNA assembly

A Casini, M Storch, GS Baldwin, T Ellis - Nature Reviews Molecular Cell …, 2015 - nature.com
DNA assembly is a key part of constructing gene expression systems and even whole
chromosomes. In the past decade, a plethora of powerful new DNA assembly methods …