Changes in the physical properties of calcium alginate gel beads under a wide range of gelation temperature conditions

C Jeong, S Kim, C Lee, S Cho, SB Kim - Foods, 2020 - mdpi.com
Until now, most studies using calcium alginate gel (CAG) have been conducted primarily at
room temperature (20° C) without considering gelation temperature. Moreover, the effects of …

Green aqueous biphasic systems containing deep eutectic solvents and sodium salts for the extraction of protein

J Pang, X Sha, Y Chao, G Chen, C Han, W Zhu, H Li… - RSC …, 2017 - pubs.rsc.org
Deep eutectic solvents (DESs), a new type of green solvents, were applied for the extraction
of proteins with aqueous biphasic systems (ABSs) in this study. The structures of the …

Purification of β-mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system

NFHA Aziz, S Abbasiliasi, HS Ng… - … of Chromatography B, 2017 - Elsevier
The partitioning of β-mannanase derived from Bacillus subtilis ATCC 11774 in aqueous two-
phase system (ATPS) was studied. The ATPS containing different molecular weight of …

Effects of cholinium‐based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors

PAM Nascimento, FP Picheli, AM Lopes… - Biotechnology …, 2019 - Wiley Online Library
Lipases are well‐known biocatalysts used in several industrial processes/applications.
Thus, as with other enzymes, changes in their surrounding environment and/or their …

Incorporation of electric fields to ionic liquids-based aqueous biphasic system for enhanced recovery of extracellular Kytococcus sedentarius TWHKC01 keratinase

PE Kee, HS Yim, A Kondo, SYW Wong, PT Chen… - Journal of the Taiwan …, 2021 - Elsevier
Background The purification of keratinase is essential to enhance the catalytic efficiency of
keratinase enzyme for industrial applications. Biphasic system incorporated with electric …

Liquid–Liquid–Liquid Aqueous Triple-Phase System Based on DES and Dipotassium Hydrogen Phosphate

J Fu, X Wu, Q Xu, Y Wang, Y Yang… - Journal of Chemical & …, 2024 - ACS Publications
Deep eutectic solvent (DES) and inorganic salt can form a salt–salt aqueous two-phase
system (ATPS) and have been widely studied recently. The DES-based ATPS extends the …

Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system

JS Tan, S Abbasiliasi, AB Ariff, HS Ng, MHA Bakar… - 3 Biotech, 2018 - Springer
This study aimed at recovery of thermostable lipase from Escherichia coli BL21 using porous
glass beads grafted with polyethylene glycol (PEG) in aqueous impregnated resins system …

Purification of lipase from Burkholderia metallica fermentation broth in a column chromatography using polymer impregnated resins

ZJ Ng, S Abbasiliasi, T Yew Joon, HS Ng… - Preparative …, 2023 - Taylor & Francis
In this work, porous glass beads grafted with polyethylene glycol (PEG) were used as an
adsorbent to purify lipase from Burkholderia metallica in column chromatography. The …

Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin

YH Chow, A Sahlini, HS Ng, JCW Lan - Bioresources and Bioprocessing, 2021 - Springer
The efficacy of alcohol/sugar aqueous biphasic (ABS) system on protein extraction was
investigated. A model protein, bovine serum albumin (BSA), was adopted to evaluate the …

Ionic liquid-assisted biphasic systems for downstream processing of fermentative enzymes and organic acids

K Tonova - Physical Sciences Reviews, 2021 - degruyter.com
Room-temperature ionic liquids (ILs) represent molten salts entirely consisting of ions,
usually a charge-stabilized organic cation and an inorganic or organic anion. ILs are liquids …