Neuroscientists today can measure activity from more neurons than ever before, and are
facing the challenge of connecting these brain-wide neural recordings to computation and …

The impact of light-sheet fluorescence microscopy (LSFM) is visible in fields as diverse as
developmental and cell biology, anatomical science, biophysics and neuroscience …

A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …

Comprehensively resolving neuronal identities in whole-brain images is a major challenge.
We achieve this in C. elegans by engineering a multicolor transgene called NeuroPAL (a …

Advanced microscopy enables us to acquire quantities of time-lapse images to visualize the
dynamic characteristics of tissues, cells or molecules. Microscopy images typically vary in …

For over a century, the neuron doctrine—which states that the neuron is the structural and
functional unit of the nervous system—has provided a conceptual foundation for …

Since the introduction of calcium imaging to monitor neuronal activity with single-cell
resolution, optical imaging methods have revolutionized neuroscience by enabling …

While isolated motor actions can be correlated with activities of neuronal networks, an
unresolved problem is how the brain assembles these activities into organized behaviors …

We report a three-dimensional microscopy technique—swept, confocally-aligned planar
excitation (SCAPE) microscopy—that allows volumetric imaging of living samples at …

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major
challenge in neuroscience. Here we demonstrate simultaneous functional imaging of …