High-throughput and high-content bioassay enables tuning of polyester nanoparticles for cellular uptake, endosomal escape, and systemic in vivo delivery of mRNA
Nanoparticle-based mRNA therapeutics hold great promise, but cellular internalization and
endosomal escape remain key barriers for cytosolic delivery. We developed a dual …
endosomal escape remain key barriers for cytosolic delivery. We developed a dual …
Surface Bio‐engineered Polymeric Nanoparticles
Surface bio‐engineering of polymeric nanoparticles (PNPs) has emerged as a cornerstone
in contemporary biomedical research, presenting a transformative avenue that can …
in contemporary biomedical research, presenting a transformative avenue that can …
Bioreducible polymeric nanoparticles containing multiplexed cancer stem cell regulating miRNAs inhibit glioblastoma growth and prolong survival
Despite our growing molecular-level understanding of glioblastoma (GBM), treatment
modalities remain limited. Recent developments in the mechanisms of cell fate regulation …
modalities remain limited. Recent developments in the mechanisms of cell fate regulation …
Nanoparticles for stem cell therapy bioengineering in glioma
H Ruiz-Garcia, K Alvarado-Estrada… - … in Bioengineering and …, 2020 - frontiersin.org
Gliomas are a dismal disease associated with poor survival and high morbidity. Current
standard treatments have reached a therapeutic plateau even after combining maximal safe …
standard treatments have reached a therapeutic plateau even after combining maximal safe …
Lysosomal Proton Buffering of Poly(ethylenimine) Measured In Situ by Fluorescent pH-Sensor Microcapsules
S Roy, D Zhu, WJ Parak, N Feliu - ACS nano, 2020 - ACS Publications
Poly (ethylenimine)(PEI) is frequently used as transfection agent for delivery of nucleic acids
to the cytosol. After endocytosis of complexes of PEI and nucleic acids, a fraction of them can …
to the cytosol. After endocytosis of complexes of PEI and nucleic acids, a fraction of them can …
Ratiometric, pH-sensitive probe for monitoring siRNA delivery
MR Herling, IJ Dmochowski - Journal of the American Chemical …, 2023 - ACS Publications
Many RNA delivery strategies require efficient endosomal uptake and release. To monitor
this process, we developed a 2′-OMe RNA-based ratiometric pH probe with a pH-invariant …
this process, we developed a 2′-OMe RNA-based ratiometric pH probe with a pH-invariant …
Nonviral polymeric nanoparticles for gene therapy in pediatric CNS malignancies
Together, medulloblastoma (MB) and atypical teratoid/rhabdoid tumors (AT/RT) represent
two of the most prevalent pediatric brain malignancies. Current treatment involves radiation …
two of the most prevalent pediatric brain malignancies. Current treatment involves radiation …
Reducible branched ester-amine quadpolymers (rBEAQs) codelivering plasmid DNA and RNA oligonucleotides enable CRISPR/Cas9 genome editing
Functional codelivery of plasmid DNA and RNA oligonucleotides in the same nanoparticle
system is challenging due to differences in their physical properties as well as their …
system is challenging due to differences in their physical properties as well as their …
Size-controlled and shelf-stable DNA particles for production of lentiviral vectors
Polyelectrolyte complex particles assembled from plasmid DNA (pDNA) and poly
(ethylenimine)(PEI) have been widely used to produce lentiviral vectors (LVVs) for gene …
(ethylenimine)(PEI) have been widely used to produce lentiviral vectors (LVVs) for gene …
Ligand-free biodegradable poly (beta-amino ester) nanoparticles for targeted systemic delivery of mRNA to the lungs
Non-viral nanoparticles (NPs) have seen heightened interest as a delivery method for a
variety of clinically relevant nucleic acid cargoes in recent years. While much of the focus …
variety of clinically relevant nucleic acid cargoes in recent years. While much of the focus …