How eukaryotic chromosomes fold inside the nucleus is an age-old question that remains
unanswered today. Early biochemical and microscopic studies revealed the existence of …

The molecular mechanisms and signalling pathways that regulate the in vitro preservation of
distinct pluripotent stem cell configurations, and their induction in somatic cells by direct …

Despite its importance, understanding the early phases of human development has been
limited by availability of human samples. The recent emergence of stem-cell-derived embryo …

Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state.
However, without a tractable experimental model, the mechanism of human PGC (hPGC) …

Primordial germ cells (PGCs), the precursors of sperm and eggs, are established in
perigastrulation-stage embryos in mammals. Signals from extra-embryonic tissues induce a …

Endogenous retroviruses (ERVs) are remnants of ancient retroviral infections, and comprise
nearly 8% of the human genome. The most recently acquired human ERV is HERVK (HML …

Mouse embryonic stem (ES) cells are isolated from the inner cell mass of blastocysts, and
can be preserved in vitro in a naive inner-cell-mass-like configuration by providing …

Image-based screening is used to measure a variety of phenotypes in cells and whole
organisms. Combined with perturbations such as RNA interference, small molecules, and …

Genetic screens are critical for the systematic identification of genes underlying cellular
phenotypes. Pooling gene perturbations greatly improves scalability but is not compatible …

Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to
mark the transcription start sites of active genes. Here, we show that H3K4me3 domains that …