In experimental evolution, laboratory-controlled conditions select for the adaptation of
species, which can be monitored in real time. Despite the current popularity of such …

Laboratory evolution studies provide fundamental biological insight through direct
observation of the evolution process. They not only enable testing of evolutionary theory and …

To estimate the number and diversity of beneficial mutations, we experimentally evolved 115
populations of Escherichia coli to 42.2° C for 2000 generations and sequenced one genome …

Adaptive laboratory evolution (ALE) has emerged as a valuable method by which to
investigate microbial adaptation to a desired environment. Here, we performed ALE to 42° C …

The quantification of spontaneous mutation rates is crucial for a mechanistic understanding
of the evolutionary process. In bacteria, traditional estimates using experimental or …

Coral reefs are rapidly declining because of widespread mass coral bleaching causing
extensive coral mortality. Elevated seawater temperatures are the main drivers of coral …

Understanding how the metabolic rates of prokaryotes respond to temperature is
fundamental to our understanding of how ecosystem functioning will be altered by climate …

A novel approach for dissecting complex traits is to experimentally evolve laboratory
populations under a controlled environment shift, resequence the resulting populations, and …

Enterococcus faecium (E. faecium) is widely used in foods and is known as a probiotic to
treat or prevent diarrhea in pets and livestock. However, the poor resistance of E. faecium to …

The temporal change of phenotypes during the adaptive process remains largely
unexplored, as do the genetic changes that affect these phenotypic changes. Here we …