Protein crystals have attracted a great deal of attention as solid biomaterials because they
have porous structures created by regular assemblies of proteins. The lattice structures of …

Protein crystallization in living cells has been observed surprisingly often as a native
assembly process during the past decades, and emerging evidence indicates that this …

Despite improvements in volumetric titer for monoclonal antibody (MAb) production
processes using Chinese hamster ovary (CHO) cells, some “difficult‐to‐express”(DTE) MAbs …

With the advent of serial X-ray crystallography on microfocus beamlines at free-electron
laser and synchrotron facilities, the demand for protein microcrystals has significantly risen …

The success of biotherapeutic development heavily relies on establishing robust production
platforms. During the manufacturing process, the protein is exposed to multiple stress …

There is growing interest in the design of protein assemblies for use in materials science
and bionanotechnology. Protein assemblies, such as cages and crystalline protein …

Low culture temperature enhances the cell‐specific productivity of Chinese hamster ovary
(CHO) cells expressing varied recombinant (r‐) proteins, but the mechanisms remain …

Endoplasmic reticulum (ER) is the largest membrane-bound compartment in all cells and
functions as a key regulator in protein biosynthesis, lipid metabolism, and calcium balance …

The potential of process crystallization for purification of a therapeutic monoclonal IgG1
antibody was studied. The purified antibody was crystallized in non‐agitated micro‐batch …

With the advance of complex biological formats such as bispecific antibodies or fusion
proteins, mammalian expression systems often show low performance. Described …