Assisted reproductive techniques in mares

K Hinrichs - Reproduction in domestic animals, 2018 - Wiley Online Library
A wide variety of assisted reproductive techniques (ART s) are available to aid in managing
aspects of equine reproduction. Embryo recovery and transfer can be used to obtain more …

[HTML][HTML] The many problems of somatic cell nuclear transfer in reproductive cloning of mammals

K Malin, O Witkowska-Piłaszewicz, K Papis - Theriogenology, 2022 - Elsevier
In 1996, when Dolly the sheep was born, a new, utopian era was expected to begin. Science
fiction and popular culture instantly threatened the public with shortly upcoming human …

[HTML][HTML] Cloning horses by somatic cell nuclear transfer: Effects of oocyte source on development to foaling

JV Cortez, K Hardwicke, J Cuervo-Arango, CG Grupen - Theriogenology, 2023 - Elsevier
The cloning of horses is a commercial reality, yet the availability of oocytes for cloned
embryo production remains a major limitation. Immature oocytes collected from abattoir …

Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern

A Gambini, M Duque Rodriguez, MB Rodríguez… - PLoS …, 2020 - journals.plos.org
Several equids have gone extinct and many extant equids are currently considered
vulnerable to critically endangered. This work aimed to evaluate whether domestic horse …

A journey through horse cloning

A Gambini, M Maserati - Reproduction, Fertility and Development, 2018 - CSIRO Publishing
Interest in equine somatic cell nuclear transfer technology has increased significantly since
the first equid clones were produced in 2003. This is demonstrated by the multiple …

Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

R Olivera, LN Moro, R Jordan, N Pallarols… - Stem cells and …, 2018 - Taylor & Francis
Introduction Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming
and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal …

In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors

R Olivera, LN Moro, R Jordan, C Luzzani, S Miriuka… - PLoS …, 2016 - journals.plos.org
The demand for equine cloning as a tool to preserve high genetic value is growing
worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first …

Embryo aggregation in pig improves cloning efficiency and embryo quality

CP Buemo, A Gambini, LN Moro, MI Hiriart… - PLoS …, 2016 - journals.plos.org
In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo
development and embryo quality by measuring blastocyst diameter and cell number, DNA …

Improves the In Vitro Developmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm

L Xu, A Mesalam, KL Lee, SH Song, I Khan… - Cellular …, 2019 - liebertpub.com
Somatic cell nuclear transfer (SCNT) is a useful technology; however, its efficiency is low. In
this study, we investigated the effects of cytoplasmic transfer into enucleated oocytes on the …

Tiger, Bengal and domestic cat embryos produced by homospecific and interspecific zona‐free nuclear transfer

LN Moro, J Jarazo, C Buemo, MI Hiriart… - … in Domestic Animals, 2015 - Wiley Online Library
Contents The aim of this study was to evaluate three different cloning strategies in the
domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by …