Elimination or mitigation of the toxic effects of chemical waste released to the environment
by industrial and urban activities relies largely on the catalytic activities of microorganisms …

Highlights•Rational and modern approaches for metabolic pathway engineering.•Tailor-
made cell factories for biofuel and value-added chemical production.•Synthetic and systems …

Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …

Metabolic engineering of microorganisms to produce desirable products on an industrial
scale can result in unbalanced cellular metabolic networks that reduce productivity and …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

Visualizing biomolecular and cellular processes inside intact living organisms is a major
goal of chemical biology. However, existing molecular biosensors, based primarily on …

The RNA-guided Cas9 nuclease from CRISPR-Cas systems has emerged as a powerful
biotechnological tool. The specificity of Cas9 can be reprogrammed to cleave desired …

The cloning of long DNA segments, especially those containing large gene clusters, is of
particular importance to synthetic and chemical biology efforts for engineering organisms …

High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …

Mechanistic insights into the role of the human microbiome in the predisposition to and
treatment of disease are limited by the lack of methods to precisely add or remove microbial …