The role of more than 40 years of improvement in protein A chromatography in the growth of the therapeutic antibody industry

GR Bolton, KK Mehta - Biotechnology progress, 2016 - Wiley Online Library
Protein A chromatography has been used as the mAb capture step in the majority of FDA
submissions. In this study, the performance of protein A chromatography, as indicated by …

Affinity monolith chromatography: A review of general principles and recent developments

S Poddar, S Sharmeen, DS Hage - Electrophoresis, 2021 - Wiley Online Library
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a
monolithic support with a biological ligand or related binding agent to isolate, enrich, or …

A direct comparison between membrane adsorber and packed column chromatography performance

C Boi, A Malavasi, RG Carbonell, G Gilleskie - Journal of Chromatography …, 2020 - Elsevier
The purpose of this work was to compare side by side the performance of packed bed and
membrane chromatography adsorption processes for protein purification. The comparison …

[HTML][HTML] Development and scale-up of oligo-dT monolithic chromatographic column for mRNA capture through understanding of base-pairing interactions

N Mencin, D Štepec, A Margon, J Vidič… - Separation and …, 2023 - Elsevier
Oligo-deoxythymidilic acid (OdT) probes conjugated to solid-phase supports exhibit high
affinity for poly-adenylated mRNA (target) through high-affinity base-pairing interactions …

Affinity monolith chromatography: A review of general principles and applications

Z Li, E Rodriguez, S Azaria, A Pekarek… - Electrophoresis, 2017 - Wiley Online Library
Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the
support is a monolith and the stationary phase is a biological‐binding agent or related …

Comparative evaluation of commercial protein A membranes for the rapid purification of antibodies

J Osuofa, SM Husson - Membranes, 2023 - mdpi.com
Protein A chromatography is ubiquitous to antibody purification. The high specificity of
Protein A for binding the Fc-region of antibodies and related products enables unmatched …

Downstream processing technologies/capturing and final purification: Opportunities for innovation, change, and improvement. A review of downstream processing …

N Singh, S Herzer - … and manufacturing of recombinant antibodies and …, 2018 - Springer
Increased pressure on upstream processes to maximize productivity has been crowned with
great success, although at the cost of shifting the bottleneck to purification. As drivers were …

Nonwoven ion-exchange membranes with high protein binding capacity for bioseparations

SM Lemma, C Boi, RG Carbonell - Membranes, 2021 - mdpi.com
This study presents the preparation and characterization of UV-grafted polybutylene
terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of …

Evaluation of novel 3D-printed monolithic adsorbers against conventional chromatography columns for the purification of c-phycocyanin from Spirulina

LCT Scorza, U Simon, M Wear, A Zouliatis, S Dimartino… - Algal Research, 2021 - Elsevier
Extraction and purification of high-grade phycobiliproteins is a multistep process that
typically involves costly chromatographic separation techniques. 3D printing approaches …

Polysulfone-graft-4′-aminobenzo-15-crown-5-ether based tandem membrane chromatography for efficient adsorptive separation of lithium isotopes

H Pei, F Yan, Z Wang, C Liu, S Hou, X Ma, J Li… - … of Chromatography A, 2019 - Elsevier
Adsorptive membrane-based chromatography can provide the high separation efficiency
common to column chromatography but at a lower working pressure. Herein, a novel …