The role of more than 40 years of improvement in protein A chromatography in the growth of the therapeutic antibody industry
Protein A chromatography has been used as the mAb capture step in the majority of FDA
submissions. In this study, the performance of protein A chromatography, as indicated by …
submissions. In this study, the performance of protein A chromatography, as indicated by …
Affinity monolith chromatography: A review of general principles and recent developments
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a
monolithic support with a biological ligand or related binding agent to isolate, enrich, or …
monolithic support with a biological ligand or related binding agent to isolate, enrich, or …
A direct comparison between membrane adsorber and packed column chromatography performance
The purpose of this work was to compare side by side the performance of packed bed and
membrane chromatography adsorption processes for protein purification. The comparison …
membrane chromatography adsorption processes for protein purification. The comparison …
[HTML][HTML] Development and scale-up of oligo-dT monolithic chromatographic column for mRNA capture through understanding of base-pairing interactions
N Mencin, D Štepec, A Margon, J Vidič… - Separation and …, 2023 - Elsevier
Oligo-deoxythymidilic acid (OdT) probes conjugated to solid-phase supports exhibit high
affinity for poly-adenylated mRNA (target) through high-affinity base-pairing interactions …
affinity for poly-adenylated mRNA (target) through high-affinity base-pairing interactions …
Affinity monolith chromatography: A review of general principles and applications
Z Li, E Rodriguez, S Azaria, A Pekarek… - Electrophoresis, 2017 - Wiley Online Library
Affinity monolith chromatography, or AMC, is a liquid chromatographic method in which the
support is a monolith and the stationary phase is a biological‐binding agent or related …
support is a monolith and the stationary phase is a biological‐binding agent or related …
Comparative evaluation of commercial protein A membranes for the rapid purification of antibodies
Protein A chromatography is ubiquitous to antibody purification. The high specificity of
Protein A for binding the Fc-region of antibodies and related products enables unmatched …
Protein A for binding the Fc-region of antibodies and related products enables unmatched …
Downstream processing technologies/capturing and final purification: Opportunities for innovation, change, and improvement. A review of downstream processing …
N Singh, S Herzer - … and manufacturing of recombinant antibodies and …, 2018 - Springer
Increased pressure on upstream processes to maximize productivity has been crowned with
great success, although at the cost of shifting the bottleneck to purification. As drivers were …
great success, although at the cost of shifting the bottleneck to purification. As drivers were …
Nonwoven ion-exchange membranes with high protein binding capacity for bioseparations
This study presents the preparation and characterization of UV-grafted polybutylene
terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of …
terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of …
Evaluation of novel 3D-printed monolithic adsorbers against conventional chromatography columns for the purification of c-phycocyanin from Spirulina
Extraction and purification of high-grade phycobiliproteins is a multistep process that
typically involves costly chromatographic separation techniques. 3D printing approaches …
typically involves costly chromatographic separation techniques. 3D printing approaches …
Polysulfone-graft-4′-aminobenzo-15-crown-5-ether based tandem membrane chromatography for efficient adsorptive separation of lithium isotopes
Adsorptive membrane-based chromatography can provide the high separation efficiency
common to column chromatography but at a lower working pressure. Herein, a novel …
common to column chromatography but at a lower working pressure. Herein, a novel …