Introduction to the theory and practice of fixation of tissues

I Eltoum, J Fredenburgh, RB Myers… - Journal of …, 2001 - Taylor & Francis
Many approaches to fixation and types of fixatives have been developed and tested over the
last-century. The mechanisms by which fixatives act to harden and preserve tissues fall into …

Crystal structure of glutamate dehydrogenase 2, a positively selected novel human enzyme involved in brain biology and cancer pathophysiology

C Dimovasili, VE Fadouloglou, A Kefala… - Journal of …, 2021 - Wiley Online Library
Introduction Mammalian glutamate dehydrogenase (hGDH1 in human cells) interconverts
glutamate to α‐ketoglutarate and ammonia while reducing NAD (P) to NAD (P) H. During …

De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin

Y Papanikolau, G Tavlas, CE Vorgias… - … Section D: Biological …, 2003 - journals.iucr.org
The purification scheme of chitinase A (ChiA) from S. marcescens has been extensively
revised. The pure enzyme crystallizes readily under new crystallization conditions. The ChiA …

General methods

C Sauter, B Lorber, A McPherson, R Giegé - 2012 - Wiley Online Library
The preparation of well diffracting crystals is a crucial step of any crystallographic project.
This chapter defines the nature of biomacromolecular crystals, sketches the theoretical …

Screening and optimization methods for nonautomated crystallization laboratories

T Bergfors - … Crystallography Protocols: Volume 1, Preparation and …, 2007 - Springer
Crystallization of biological macromolecules is becoming increasingly automated. However,
for various reasons, many laboratories still perform at least some aspects of the work …

Understanding the crystallisation of an acidic protein by dilution in the ternary NaCl–2-methyl-2, 4-pentanediol–H2O system

L Costenaro, G Zaccai, C Ebel - Journal of crystal growth, 2001 - Elsevier
Halophilic malate dehydrogenase is a negatively charged protein that crystallises well in a
dilution process following a complex interplay with the three components of NaCl–MPD …

Crystallization of type I chloramphenicol acetyltransferase: an approach based on the concept of ionic strength reducers

AE Andreeva, BE Borissova, R Mironova… - … Section D: Biological …, 2000 - journals.iucr.org
Chloramphenicol acetyltransferase (CAT) is responsible for bacterial resistance to
chloramphenicol. It catalyzes inactivation of the antibiotic by acetyl-group transfer from acetyl …

Ionic strength reducers: an efficient approach to protein purification and crystallization. Application to two Rop variants

Y Papanikolau, D Kotsifaki… - … Section D: Biological …, 2004 - journals.iucr.org
Detailed knowledge of the influence of various parameters on macromolecular solubility is
essential for crystallization. The concept of so-calledionic strength reducers' provides insight …

Improving marginal crystals

CW Carter, M Riès-Kautt - … : Volume 1, Preparation and Crystallization of …, 2007 - Springer
The physical chemistry of crystal growth can help to identify directions in which to look for
improved crystal properties. In this chapter, we summarize how crystal growth depends on …

Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol

M Wan, MD Chavez, JL Schrimsher - US Patent 7,998,705, 2011 - Google Patents
Ion exchange chromatography is one of the most widely used methods for separating,
identifying and/or quantifying amounts of proteins and/or peptides in a mixture or solution …