The molecular basis and disease relevance of non-homologous DNA end joining

B Zhao, E Rothenberg, DA Ramsden… - … Reviews Molecular Cell …, 2020 - nature.com
Non-homologous DNA end joining (NHEJ) is the predominant repair mechanism of any type
of DNA double-strand break (DSB) during most of the cell cycle and is essential for the …

Structural insights into the evolution of the RAG recombinase

C Liu, Y Zhang, CC Liu, DG Schatz - Nature Reviews Immunology, 2022 - nature.com
Adaptive immunity in jawed vertebrates relies on the assembly of antigen receptor genes by
the recombination activating gene 1 (RAG1)–RAG2 (collectively RAG) recombinase in a …

CryoDRGN: reconstruction of heterogeneous cryo-EM structures using neural networks

ED Zhong, T Bepler, B Berger, JH Davis - Nature methods, 2021 - nature.com
Cryo-electron microscopy (cryo-EM) single-particle analysis has proven powerful in
determining the structures of rigid macromolecules. However, many imaged protein …

Design powerful predictor for mRNA subcellular location prediction in Homo sapiens

ZY Zhang, YH Yang, H Ding, D Wang… - Briefings in …, 2021 - academic.oup.com
Messenger RNAs (mRNAs) shoulder special responsibilities that transmit genetic code from
DNA to discrete locations in the cytoplasm. The locating process of mRNA might provide …

The recent advances in non-homologous end-joining through the lens of lymphocyte development

XS Wang, BJ Lee, S Zha - DNA repair, 2020 - Elsevier
Lymphocyte development requires ordered assembly and subsequent modifications of the
antigen receptor genes through V (D) J recombination and Immunoglobulin class switch …

[HTML][HTML] How DNA loop extrusion mediated by cohesin enables V (D) J recombination

JM Peters - Current opinion in cell biology, 2021 - Elsevier
Abstract 'Structural maintenance of chromosomes'(SMC) complexes are required for the
folding of genomic DNA into loops. Theoretical considerations and single-molecule …

RAG2 abolishes RAG1 aggregation to facilitate V (D) J recombination

T Gan, Y Wang, Y Liu, DG Schatz, J Hu - Cell reports, 2021 - cell.com
RAG1 and RAG2 form a tetramer nuclease to initiate V (D) J recombination in developing T
and B lymphocytes. The RAG1 protein evolves from a transposon ancestor and possesses …

Analysis of IGH allele content in a sample group of rheumatoid arthritis patients demonstrates unrevealed population heterogeneity

U Hardt, MM Corcoran, S Narang… - Frontiers in …, 2023 - frontiersin.org
Immunoglobulin heavy chain (IGH) germline gene variations influence the B cell receptor
repertoire, with resulting biological consequences such as shaping our response to …

Cutting antiparallel DNA strands in a single active site

X Chen, Y Cui, RB Best, H Wang, ZH Zhou… - Nature structural & …, 2020 - nature.com
A single enzyme active site that catalyzes multiple reactions is a well-established
biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix …

Molecular mechanisms of DNA sequence selectivity in V (D) J recombination

W Hoolehan, JC Harris, KK Rodgers - ACS omega, 2023 - ACS Publications
Antigen receptor (AgR) diversity is central to the ability of adaptive immunity in jawed
vertebrates to protect against pathogenic agents. The production of highly diverse AgR …