The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …

Background Technologies for making and manipulating DNA have enabled advances in
biology ever since the discovery of the DNA double helix. But introducing site-specific …

The CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-
associated proteins) modules are adaptive immunity systems that are present in many …

Background All archaeal and many bacterial genomes contain Clustered Regularly
Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR …

Methods for multiplex amplification of a plurality of targets of distinct sequence from a
complex mixture are disclosed. In one aspect targets are circularized using a single …

CRISPR/Cas9 has emerged as a simple, precise and most rapid genome editing
technology. With a number of promising applications ranging from agriculture and …

Mutations in mammalian and Drosophila Hel308 and PolQ paralogues cause genome
instability but their helicase functions are mysterious. By in vivo and in vitro analysis, we …

Gene therapy using CRISPR Cas9 technique is rapidly gaining popularity among the
scientific community primarily because of its versatility, cost-effectiveness, and high efficacy …

Prokaryotes are constantly coping with attacks by viruses in their natural environments and
therefore have evolved an impressive array of defense systems. Clustered Regularly …

CRISPR (clustered regularly interspaced short palindromic repeat) systems provide bacteria
and archaea with adaptive immunity to repel invasive genetic elements. Type I systems use …