ATPases associated with diverse cellular activities (AAA+ proteins) are a superfamily of
proteins found throughout all domains of life. The hallmark of this family is a conserved …

Multi-protein DNA replication complexes called replisomes perform the essential process of
copying cellular genetic information prior to cell division. Under ideal conditions, replisomes …

Abstract In Escherichia coli, the DnaB helicase forms the basis for the assembly of the DNA
replication complex. The stability of DnaB at the replication fork is likely important for …

In cells, dedicated AAA+ ATPases deposit hexameric, ring-shaped helicases onto DNA to
initiate chromosomal replication. To better understand the mechanisms by which helicase …

To initiate DNA replication, it is essential to properly assemble a pair of replicative helicases
at each replication origin. While the general principle of this process applies universally from …

Highlights•Cryo-EM structures of Escherichia coli DNA polymerase III complexes.•Bacterial
polymerases quickly exchange at replication forks.•Bacterial replisomes can work in a …

The initiation of chromosomal DNA replication starts at a replication origin, which in bacteria
is a discrete locus that contains DNA sequence motifs recognized by an initiator protein …

Unwinding of the replication origin and loading of DNA helicases underlie the initiation of
chromosomal replication. In Escherichia coli, the minimal origin oriC contains a duplex …

Initiation of DNA replication is required for cell viability and passage of genetic information to
the next generation. Studies in Escherichia coli and Bacillus subtilis have established A …

The DNA replication protein DnaA in Escherichia coli constructs higher-order complexes on
the origin, oriC, to unwind this region. DnaB helicase is loaded onto unwound oriC via …