CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …

Functional enrichment analysis is pivotal for interpreting high-throughput omics data in life
science. It is crucial for this type of tool to use the latest annotation databases for as many …

The engineering of autologous patient T cells for adoptive cell therapies has revolutionized
the treatment of several types of cancer. However, further improvements are needed to …

The immune-suppressive tumour microenvironment represents a major obstacle to effective
immunotherapy,. Pathologically activated neutrophils, also known as polymorphonuclear …

Generation of functionally mature organs requires exquisite control of transcriptional
programs governing cell state transitions during development. Despite advances in …

How the KRAS oncogene drives cancer growth remains poorly understood. Therefore, we
established a systemwide portrait of KRAS-and extracellular signal–regulated kinase (ERK) …

Nanotechnology provides platforms to deliver medical agents to specific cells. However, the
nanoparticle's surface becomes covered with serum proteins in the blood after …

Immune checkpoint blockade (ICB) therapy revolutionized cancer treatment, but many
patients with impaired MHC-I expression remain refractory. Here, we combined FACS-based …

It is estimated that only 0.02% of disseminated tumour cells are able to seed overt
metastases. While this suggests the presence of environmental constraints to metastatic …