Limited understanding of human proteoforms with complex posttranslational modifications
and the underlying mechanisms poses a major obstacle to research on human health and …

The ability to manipulate the chemical composition of proteins and peptides has been
central to the development of improved polypeptide-based therapeutics and has enabled …

The chemical synthesis of homogeneously modified histones is a powerful approach to
quantitatively decipher how post-translational modifications (PTMs) modulate epigenetic …

The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

We have described the chemical synthesis of d-Sortase A in large quantity and high purity
by a hydrazide ligation strategy. The d-Sortase was fully active toward d-peptides and D/L …

Differentiation between similarly reactive sites in molecules represents an ongoing
challenge of organic synthesis. Herein we described one kind of versatile reagents, N …

Ferricyanide-promoted oxidative activation of N-acylated α-aminothioacids for amide bond
formation with α-aminonitriles was recently shown to be a plausible pathway for prebiotic …

Site-specific protein labeling plays important roles in drug discovery and illuminating
biological processes at the molecular level. However, it is challenging to label proteins with …

Bacteria have evolved a diverse set of enzymes that enable them to subvert host defense
mechanisms as well as to form part of the prokaryotic immune system. Due to their unique …