Control of the oxygen dependence of an implantable polymer/enzyme composite biosensor for glutamate

CP McMahon, G Rocchitta, PA Serra… - Analytical …, 2006 - ACS Publications
Biosensors for glutamate (Glu) were fabricated from Teflon-coated Pt wire (cylinders and
disks), modified with the enzyme glutamate oxidase (GluOx) and electrosynthesized polymer …

Mathematical analysis of reaction–diffusion equations modeling the michaelis–menten kinetics in a micro-disk biosensor

NA Khan, FS Alshammari, CAT Romero, M Sulaiman… - Molecules, 2021 - mdpi.com
In this study, we have investigated the mathematical model of an immobilized enzyme
system that follows the Michaelis–Menten (MM) kinetics for a micro-disk biosensor. The film …

Oxygen tolerance of an implantable polymer/enzyme composite glutamate biosensor displaying polycation-enhanced substrate sensitivity

CP McMahon, G Rocchitta, SM Kirwan… - Biosensors and …, 2007 - Elsevier
Biosensors were fabricated at neutral pH by sequentially depositing the polycation
polyethyleneimine (PEI), the stereoselective enzyme l-glutamate oxidase (GluOx) and the …

Reaction/diffusion equation with Michaelis-Menten kinetics in microdisk biosensor: Homotopy perturbation method approach

R Swaminathan, KL Narayanan, V Mohan… - International Journal of …, 2019 - Elsevier
This paper presents the non steady state model of a microdisk enzyme based biosensor
where the enzyme reacts directly on the electrode itself. The model is based on diffusion …

Glucose diffusivity in cell culture medium

H Suhaimi, S Wang, DB Das - Chemical Engineering Journal, 2015 - Elsevier
The diaphragm cell method (DCM) was applied to determine the self-diffusion of glucose in
cell culture media (CCM) and water (reference fluid). For this purpose, a diffusion cell based …

Mathematical model of whole cell based bio-chip: An electrochemical biosensor for water toxicity detection

R Popovtzer, A Natan, Y Shacham-Diamand - Journal of Electroanalytical …, 2007 - Elsevier
Whole cell biosensors, which have been genetically engineered to respond to
environmental stress, trigger a sequence of processes, which leads to generation of …

The efficiency of immobilised glutamate oxidase decreases with surface enzyme loading: an electrostatic effect, and reversal by a polycation significantly enhances …

CP McMahon, G Rocchitta, PA Serra, SM Kirwan… - Analyst, 2006 - pubs.rsc.org
The apparent Michaelis constant, KM, for glutamate oxidase (GluOx) immobilised on Pt
electrodes increased systematically with enzyme loading. The effect was due, at least in …

A Detailed Model of Electroenzymatic Glutamate Biosensors To Aid in Sensor Optimization and in Applications in Vivo

M Clay, HG Monbouquette - ACS chemical neuroscience, 2018 - ACS Publications
Simulations conducted with a detailed model of glutamate biosensor performance describe
the observed sensor performance well, illustrate the limits of sensor performance, and …

Design variations of a polymer–enzyme composite biosensor for glucose: Enhanced analyte sensitivity without increased oxygen dependence

CP McMahon, SJ Killoran, RD O'Neill - Journal of Electroanalytical …, 2005 - Elsevier
The glucose sensitivity and oxygen dependence of a variety of implantable biosensors
based on glucose oxidase (GOx), incorporating an electrosynthesized poly-o …

On glucose diffusivity of tissue engineering membranes and scaffolds

H Suhaimi, S Wang, T Thornton, DB Das - Chemical Engineering Science, 2015 - Elsevier
There has been an increasing interest in the concept of growing artificial tissues in
bioreactors which use numerous membranes and scaffolds to support the cellular processes …