Modern light microscopy, including super-resolution techniques, has brought about a
demand for small labeling tags that bring the fluorophore closer to the target. This challenge …

Aligned with the increasing importance of bioorthogonal chemistry has been an increasing
demand for more potent, affordable, multifunctional, and programmable bioorthogonal …

Engineering new functionality into living eukaryotic systems by enzyme evolution or de novo
protein design is a formidable challenge. Cells do not rely exclusively on DNA-based …

Genetic code expansion has emerged as an enabling tool to provide insight into functions of
understudied proteinogenic species, such as small proteins and peptides, and to probe …

Quantitative labeling of biomolecules is necessary to advance areas of antibody–drug
conjugation, super-resolution microscopy imaging of molecules in live cells, and …

Despite a range of covalent protein modifications, few techniques exist for quantification of
protein bioconjugation in cells. Here, we describe a novel method for quantifying in cellulo …

Fluorogenic labeling via bioorthogonal tetrazine chemistry has proven to be highly
successful in fluorescence microscopy of living cells. To date, trans-cyclooctene (TCO) and …

Bioconjugation reactions are critical to the modification of peptides and proteins, permitting
the introduction of biophysical probes onto proteins as well as drugs for use in antibody …

Due to their target specificity, antibody–drug conjugates─ monoclonal antibodies
conjugated to a cytotoxic moiety─ are efficient therapeutics that can kill malignant cells …

Membraneless organelles are capable of selectively performing complex tasks in living cells
despite dynamically exchanging with their surroundings. This is an exquisite example how …