Antimicrobial resistance (AMR) is a serious threat to global public health. It increases
morbidity and mortality, and is associated with high economic costs due to its health care …

Viruses and their hosts are engaged in a constant arms race leading to the evolution of
antiviral defence mechanisms. Recent studies have revealed that the immune arsenal of …

Microbial biocontainment is an essential goal for engineering safe, next-generation living
therapeutics. However, the genetic stability of biocontainment circuits, including kill switches …

Phages differ substantially in the bacterial hosts that they infect. Their host range is
determined by the specific structures that they use to target bacterial cells. Tailed phages …

Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …

CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and
prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to …

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …

Abstract Development of hyperproducing strains is important for biomanufacturing of
biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

Antibiotic resistance threatens our ability to treat infectious diseases, spurring interest in
alternative antimicrobial technologies. The use of bacterial conjugation to deliver CRISPR …