[HTML][HTML] Bacterial vaginosis: current diagnostic avenues and future opportunities

MJ Redelinghuys, J Geldenhuys, H Jung… - Frontiers in cellular and …, 2020 - frontiersin.org
A healthy female genital tract harbors a microbiome dominated by lactic acid and hydrogen
peroxide producing bacteria, which provide protection against infections by maintaining a …

[HTML][HTML] The future agricultural biogas plant in Germany: A vision

S Theuerl, C Herrmann, M Heiermann, P Grundmann… - Energies, 2019 - mdpi.com
After nearly two decades of subsidized and energy crop-oriented development, agricultural
biogas production in Germany is standing at a crossroads. Fundamental challenges need to …

Primer, pipelines, parameters: issues in 16S rRNA gene sequencing

I Abellan-Schneyder, MS Matchado, S Reitmeier… - Msphere, 2021 - Am Soc Microbiol
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

[HTML][HTML] Interpretations of environmental microbial community studies are biased by the selected 16S rRNA (gene) amplicon sequencing pipeline

D Straub, N Blackwell, A Langarica-Fuentes… - Frontiers in …, 2020 - frontiersin.org
One of the major methods to identify microbial community composition, to unravel microbial
population dynamics, and to explore microbial diversity in environmental samples is high …

The newest Oxford Nanopore R10. 4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling

T Zhang, H Li, S Ma, J Cao, H Liao… - Applied and …, 2023 - Am Soc Microbiol
The long-read amplicon provides a species-level solution for the community. With the
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …

[HTML][HTML] A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes

AB Nygaard, HS Tunsjø, R Meisal, C Charnock - Scientific Reports, 2020 - nature.com
There is a growing awareness of the importance of indoor microbiomes for human health.
Given their complexity, these microbiomes can only be adequately surveyed using high …

[HTML][HTML] Freshwater monitoring by nanopore sequencing

L Urban, A Holzer, JJ Baronas, MB Hall… - Elife, 2021 - elifesciences.org
While traditional microbiological freshwater tests focus on the detection of specific bacterial
indicator species, including pathogens, direct tracing of all aquatic DNA through …

Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges

A Devi, YT Chiu, HT Hsueh, TF Lin - Water research, 2021 - Elsevier
Taste and odor (T&O) are an important issue in drinking water, aquaculture, recreation and a
few other associated industries, and cyanobacteria-relevant geosmin and 2 …

[HTML][HTML] Does intraspecific variation in rDNA copy number affect analysis of microbial communities?

A Lavrinienko, T Jernfors, JJ Koskimäki, AM Pirttilä… - Trends in …, 2021 - cell.com
Amplicon sequencing of partial regions of the ribosomal RNA loci (rDNA) is widely used to
profile microbial communities. However, the rDNA is dynamic and can exhibit substantial …

Relative performance of MinION (Oxford Nanopore Technologies) versus Sequel (Pacific Biosciences) third-generation sequencing instruments in identification of …

K Loit, K Adamson, M Bahram, R Puusepp… - Applied and …, 2019 - Am Soc Microbiol
Culture-based molecular identification methods have revolutionized detection of pathogens,
yet these methods are slow and may yield inconclusive results from environmental …