Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories

SW Jung, J Yeom, JS Park, SM Yoo - Biotechnology Advances, 2021 - Elsevier
To overcome environmental problems caused by the use of fossil resources, microbial cell
factories have become a promising technique for the sustainable and eco-friendly …

Escherichia coli as a platform microbial host for systems metabolic engineering

D Yang, CPS Prabowo, H Eun, SY Park… - Essays in …, 2021 - portlandpress.com
Bio-based production of industrially important chemicals and materials from non-edible and
renewable biomass has become increasingly important to resolve the urgent worldwide …

Engineering Halomonas bluephagenesis via small regulatory RNAs

LJ Wang, XR Jiang, J Hou, CH Wang, GQ Chen - Metabolic Engineering, 2022 - Elsevier
Halomonas bluephagenesis, a robust and contamination-resistant microorganism has been
developed as a chassis for “Next Generation Industrial Biotechnology”. The non-model H …

Recent advances in high-throughput metabolic engineering: Generation of oligonucleotide-mediated genetic libraries

Y Li, EO Mensah, E Fordjour, J Bai, Y Yang… - Biotechnology Advances, 2022 - Elsevier
The preparation of genetic libraries is an essential step to evolve microorganisms and study
genotype-phenotype relationships by high-throughput screening/selection. As the large …

[HTML][HTML] Engineering microorganisms to produce bio-based monomers: progress and challenges

C Chen, X Chen, L Liu, J Wu, C Gao - Fermentation, 2023 - mdpi.com
Bioplastics are polymers made from sustainable bio-based feedstocks. While the potential of
producing bio-based monomers in microbes has been investigated for decades, their …

Recent advances in systems metabolic engineering strategies for the production of biopolymers

YJ Sohn, HT Kim, SY Jo, HM Song, KA Baritugo… - Biotechnology and …, 2020 - Springer
Biopolymers consisting of at least one monomer, which are produced from renewable
carbon sources, are being highly sought out since ubiquitous plastics are mainly produced …

[HTML][HTML] Extremely low leakage expression systems using dual transcriptional-translational control for toxic protein production

Y Kato - International Journal of Molecular Sciences, 2020 - mdpi.com
Expression systems for highly toxic protein genes must be conditional and suppress leakage
expression to almost zero because even faint leakage expression may kill host cells, inhibit …

Synthetic small regulatory RNAs in microbial metabolic engineering

WH Xie, HK Deng, J Hou, LJ Wang - Applied Microbiology and …, 2021 - Springer
Small regulatory RNAs (sRNAs) finely control gene expression in prokaryotes and synthetic
sRNA has become a useful high-throughput approach to tackle current challenges in …

微生物中一碳代谢网络构建的进展与挑战

郭姝媛, 吴良焕, 刘香健, 王博, 于涛 - 合成生物学, 2022 - synbioj.cip.com.cn
利用来源广, 价格低, 易制备且储量丰富的一碳化合物作为底物, 通过构建甲基营养型细胞工厂,
生物合成多种高附加值化学品, 不仅可以促进一碳资源的洁净利用, 同时可以缓解能源短缺 …

[HTML][HTML] Development of a DNA double-strand break-free base editing tool in Corynebacterium glutamicum for genome editing and metabolic engineering

C Deng, X Lv, J Li, Y Liu, G Du, L Liu - Metabolic Engineering …, 2020 - Elsevier
As a traditional amino acid producing bacterium, Corynebacterium glutamicum is a platform
strain for production of various fine chemicals. Based on the CRISPR (Clustered regularly …