Maternal age affects pronuclear and chromatin dynamics, morula compaction and cell polarity, and blastulation of human embryos

K Ezoe, T Miki, H Akaike, K Shimazaki… - Human …, 2023 - academic.oup.com
STUDY QUESTION Does maternal ageing impact early and late morphokinetic and cellular
processes of human blastocyst formation? SUMMARY ANSWER Maternal ageing …

Human 1PN and 3PN zygotes recapitulate all morphokinetic events of normal fertilization but reveal novel developmental errors

K Ezoe, T Takahashi, K Shimazaki, T Miki… - Human …, 2022 - academic.oup.com
Abstract STUDY QUESTION Does mono-(1PN) and tri-pronuclear (3PN) fertilization
recapitulate the morphokinetic changes of normal bi-pronuclear (2PN) fertilization …

Developmental perturbation in human embryos: Clinical and biological significance learned from time‐lapse images

K Ezoe, T Takahashi, T Miki… - Reproductive Medicine and …, 2024 - Wiley Online Library
Background Time‐lapse technology (TLT) has gained widespread adoption worldwide. In
addition to facilitating the undisturbed culture of embryos, TLT offers the unique capability of …

Correlation between female pronuclear/cytoplasmic ratio and number of chromosomes in mouse zygotic stage: implications for aneuploidy assessment in ART

W Xiao, S Akao, J Otsuki - Journal of Assisted Reproduction and Genetics, 2024 - Springer
Purpose The objective of this study was to investigate the correlation between the
pronuclear/cytoplasmic (PN/C) ratio and the number of chromosomes in mouse zygotes to …

Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes

N Yonezawa, T Shindo, H Oda, H Kimura… - Genes to …, 2024 - Wiley Online Library
In eukaryotes, DNA is housed within the cell nucleus. Molecules required for the formation of
a nucleus have been identified using in vitro systems with frog egg extracts and in vivo …

[PDF][PDF] Study on determinants of pronuclear size: cytoplasmic volume and chromosome number

WEI XIAO - 2024 - ousar.lib.okayama-u.ac.jp
Background: Pronuclear-to-cytoplasmic (PN/C) ratio and morphological control mechanism
of the pronucleus in mammalian zygotes are critical for understanding zygote development …

[HTML][HTML] Developmental trajectory of monopronucleated zygotes after in vitro fertilization when they include both male and female genomes

X Wei, N Enatsu, K Furuhashi, T Iwasaki, S Kokeguchi… - Fertility and …, 2022 - Elsevier
Objective To examine the cause of monopronucleated zygote (1PN) formation that includes
both maternal and paternal genomes. Design Retrospective cohort study. Setting Private …

[PDF][PDF] Advanced maternal age (AMA)–der mütterliche Alterseffekt beginnt schon im Vorkernstadium

H sind zum einen … - Gynäkologische …, 2023 - dggef.de
Der reproduktive Erfolg hängt in hohem Maße vom Alter der beiden Partner ab, wobei das
mütterliche Alter sicherlich den größten Einfluss hat. So sinkt mit zunehmendem …

[PDF][PDF] 体外受精における妊娠率向上に寄与する要因および不妊原因に関する研究

X WEI - 2023 - ousar.lib.okayama-u.ac.jp
我が国における出産児の 14 人に 1 人が体外受精により産まれており, 累計で 70 万人を突破した
(2019 年, 厚生労働省). 体外受精では通常 1 前核 (1PN) 形成や 3 つ以上の前核形成は異常受精 …