The Rosetta software for macromolecular modeling, docking and design is extensively used
in laboratories worldwide. During two decades of development by a community of …

The computational de novo protein design is increasingly applied to address a number of
key challenges in biomedicine and biological engineering. Successes in expanding …

Background CCR5 is the major coreceptor for human immunodeficiency virus (HIV). We
investigated whether site-specific modification of the gene (“gene editing”)—in this case, the …

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …

Recent developments in computational chemistry and biology have come together in the
“inside‐out” approach to enzyme engineering. Proteins have been designed to catalyze …

Macromolecular modeling and design are increasingly useful in basic research,
biotechnology, and teaching. However, the absence of a user-friendly modeling framework …

Genome editing driven by zinc-finger nucleases (ZFNs) yields high gene-modification
efficiencies (> 10%) by introducing a recombinogenic double-strand break into the targeted …

The importance of safer approaches for gene therapy has been underscored by a series of
severe adverse events (SAEs) observed in patients involved in clinical trials for Severe …

Advances over the past few years have begun to enable prediction and design of
macromolecular structures at near-atomic accuracy. Progress has stemmed from the …

We describe RosettaRemodel, a generalized framework for flexible protein design that
provides a versatile and convenient interface to the Rosetta modeling suite …