[HTML][HTML] Isothermal amplification of nucleic acids: The race for the next “gold standard”

BB Oliveira, B Veigas, PV Baptista - Frontiers in Sensors, 2021 - frontiersin.org
Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …

Methods in virus diagnostics: from ELISA to next generation sequencing

N Boonham, J Kreuze, S Winter, R van der Vlugt… - Virus research, 2014 - Elsevier
Despite the seemingly continuous development of newer and ever more elaborate methods
for detecting and identifying viruses, very few of these new methods get adopted for routine …

[HTML][HTML] Phytophthora: an ancient, historic, biologically and structurally cohesive and evolutionarily successful generic concept in need of preservation

C Brasier, B Scanu, D Cooke, T Jung - IMA fungus, 2022 - Springer
The considerable economic and social impact of the oomycete genus Phytophthora is well
known. In response to evidence that all downy mildews (DMs) reside phylogenetically within …

Onsite detection of plant viruses using isothermal amplification assays

AI Bhat, R Aman, M Mahfouz - Plant biotechnology journal, 2022 - Wiley Online Library
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

[HTML][HTML] Advanced DNA-based point-of-care diagnostic methods for plant diseases detection

HY Lau, JR Botella - Frontiers in plant science, 2017 - frontiersin.org
Diagnostic technologies for the detection of plant pathogens with point-of-care capability
and high multiplexing ability are an essential tool in the fight to reduce the large agricultural …

Development of LAMP and Real-Time PCR Methods for the Rapid Detection of Xylella fastidiosa for Quarantine and Field Applications

SJ Harper, LI Ward, GRG Clover - Phytopathology, 2010 - Am Phytopath Society
Xylella fastidiosa is a regulated plant pathogen in many parts of the world. To increase
diagnostic capability of X. fastidiosa in the field, a loop-mediated isothermal amplification …

Identification and Detection of Phytophthora: Reviewing Our Progress, Identifying Our Needs

FN Martin, ZG Abad, Y Balci, K Ivors - Plant disease, 2012 - Am Phytopath Society
With the increased attention given to the genus Phytophthora in the last decade in response
to the ecological and economic impact of several invasive species (such as P. ramorum, P …

[HTML][HTML] Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

M Jauset-Rubio, M Svobodová, T Mairal, C McNeil… - Scientific reports, 2016 - nature.com
Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-
of-need are critical for the emerging field of personalised medicine for which companion …

Molecular tools for detection of plant pathogenic fungi and fungicide resistance

N Capote, AM Pastrana, A Aguado… - Plant …, 2012 - books.google.com
Plant pathogenic fungi are the causal agents of the most detrimental diseases in plants,
including economically important crops, provoking considerable yield losses worldwide …

Relative performance of MinION (Oxford Nanopore Technologies) versus Sequel (Pacific Biosciences) third-generation sequencing instruments in identification of …

K Loit, K Adamson, M Bahram, R Puusepp… - Applied and …, 2019 - Am Soc Microbiol
Culture-based molecular identification methods have revolutionized detection of pathogens,
yet these methods are slow and may yield inconclusive results from environmental …