Genomics in the long-read sequencing era

EL van Dijk, D Naquin, K Gorrichon, Y Jaszczyszyn… - Trends in Genetics, 2023 - cell.com
Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …

Plant pan-genomics and its applications

J Shi, Z Tian, J Lai, X Huang - Molecular Plant, 2023 - cell.com
Plant genomes are so highly diverse that a substantial proportion of genomic sequences are
not shared among individuals. The variable DNA sequences, along with the conserved core …

TBtools-II: A “one for all, all for one” bioinformatics platform for biological big-data mining

C Chen, Y Wu, J Li, X Wang, Z Zeng, J Xu, Y Liu… - Molecular plant, 2023 - cell.com
Since the official release of the stand-alone bioinformatics toolkit TBtools in 2020, its
superior functionality in data analysis has been demonstrated by its widespread adoption by …

A complete telomere-to-telomere assembly of the maize genome

J Chen, Z Wang, K Tan, W Huang, J Shi, T Li, J Hu… - Nature …, 2023 - nature.com
A complete telomere-to-telomere (T2T) finished genome has been the long pursuit of
genomic research. Through generating deep coverage ultralong Oxford Nanopore …

Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing

M Alonge, L Lebeigle, M Kirsche, K Jenike, S Ou… - Genome biology, 2022 - Springer
Advancing crop genomics requires efficient genetic systems enabled by high-quality
personalized genome assemblies. Here, we introduce RagTag, a toolset for automating …

Complete genomic and epigenetic maps of human centromeres

N Altemose, GA Logsdon, AV Bzikadze, P Sidhwani… - Science, 2022 - science.org
Existing human genome assemblies have almost entirely excluded repetitive sequences
within and near centromeres, limiting our understanding of their organization, evolution, and …

A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

Y Deng, S Liu, Y Zhang, J Tan, X Li, X Chu, B Xu… - Molecular plant, 2022 - cell.com
Watermelon, Citrullus lanatus, is the world's third largest fruit crop. Reference genomes with
gaps and a narrow genetic base hinder functional genomics and genetic improvement of …

The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding

X Shi, S Cao, X Wang, S Huang, Y Wang… - Horticulture …, 2023 - academic.oup.com
Grapevine is one of the most economically important crops worldwide. However, the
previous versions of the grapevine reference genome tipically consist of thousands of …

Evolutionary analysis of a complete chicken genome

Z Huang, Z Xu, H Bai, Y Huang… - Proceedings of the …, 2023 - National Acad Sciences
Microchromosomes are prevalent in nonmammalian vertebrates [PD Waters et al., Proc.
Natl. Acad. Sci. USA 118 (2021)], but a few of them are missing in bird genome assemblies …

quarTeT: a telomere-to-telomere toolkit for gap-free genome assembly and centromeric repeat identification

Y Lin, C Ye, X Li, Q Chen, Y Wu, F Zhang… - Horticulture …, 2023 - academic.oup.com
A high-quality genome is the basis for studies on functional, evolutionary, and comparative
genomics. The majority of attention has been paid to the solution of complex chromosome …