The parallel reaction monitoring (PRM) assay has emerged as an alternative method of
targeted quantification. The PRM assay is performed in a high resolution and high mass …

Highlights•Overview on available covalent warheads.•Design principles of covalent
fragment libraries.•Surrogate assay methodologies for testing reactivity.•Covalent screening …

Autophagy captures intracellular components and delivers them to lysosomes, where they
are degraded and recycled to sustain metabolism and to enable survival during starvation …

Targeting bromodomains (BRDs) of the bromo-and-extra-terminal (BET) family offers
opportunities for therapeutic intervention in cancer and other diseases. Here, we profile the …

Mammalian mtDNA encodes only 13 proteins, all essential components of respiratory
complexes, synthesized by mitochondrial ribosomes. Mitoribosomes contain greatly …

Atopic dermatitis (AD) is a complex multifactorial inflammatory skin disease that affects~ 280
million people worldwide. About 85% of AD cases begin in childhood, a significant portion of …

Background The purpose of this project was to identify metabolites, proteins, genes, and
promoters associated with water stress responses in soybean. A number of these may serve …

Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2
conjugating proteins, consisting of the determination of 15 new high-resolution three …

Protein–protein interactions (PPIs) between modular binding domains and their target
peptide motifs are thought to largely depend on the intrinsic binding specificities of the …

The human vitreous contains primarily water, but also contains proteins which have yet to be
fully characterized. To gain insight into the four vitreous substructures and their potential …