DNA hydrolysis mediated by di-and multi-nuclear metal complexes is of increasing
importance in biotechnology and medicine. Many systems for enzyme-mediated nucleic acid …

Ribozymes can catalyze a large variety of different reactions, but very few mechanistic
aspects are understood in detail. 1-9 It is evident that RNAs are always associated with …

Restriction endonucleases are enzymes which recognize short DNA sequences and cleave
the DNA in both strands. Depending on the enzymological properties different types are …

Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past
year. This doubles the number of restriction endonuclease structures now known from two to …

Type II restriction enzymes are characterized by their remarkable specificity and simplicity.
They require only divalent metals (such as Mg 2+ or Mn 2+) as cofactors to catalyze the …

DEFECTS in human DNA repair proteins can give rise to the autosomal recessive disorders
xeroderma pigmentosum (XP) and Cockayne's syndrome (CS), sometimes even together1 …

The invention provides methods, compositions and kits for segregating a target nucleic acid
from a mixed nucleic acid sample. The methods, compositions and kits comprise a non …

TYPE II restriction endonucleases are characterized by the remarkable specificity with which
they cleave specific DNA sequences. Surprisingly, their protein sequences are in most …

The catalytic mechanisms of type II restriction endonucleases and homing endonucleases
are discussed and compared. Brief reviews of the chemistry of phosphoryl transfers and …

(A) BC excinuclease of Escherichia coli removes damaged nucleotides from DNA by
hydrolyzing the 8th phosphodiester bond 5'and the 15th phosphodiester bond 3'to the …