The light-oxygen-voltage (LOV) domains of phototropins emerged as essential constituents
of light-sensitive proteins, helping initiate blue light-triggered responses. Moreover, these …

This review aims to systematize data on the construction and applications of bacterial lux-
biosensors in various fields ranging from investigation of gene regulation and regulatory …

Photoenzymatic reactions involving flavin-dependent" ene"-reductases (EREDs) rely on
protein-templated charge transfer (CT) complexes between the cofactor and substrate for …

Singlet oxygen generation has long been considered the key feature that allows genetically
encoded fluorescent tags to produce polymeric contrast agents for electron microscopy …

The field of microbiology traditionally focuses on studying microorganisms at the population
level. Nevertheless, the application of single-cell level methods, including microfluidics and …

Flavin mononucleotide-binding proteins or domains emit cyan-green fluorescence under
aerobic and anaerobic conditions, but relatively low fluorescence and less thermostability …

Recent advances in machine learning techniques have led to development of a number of
protein design and engineering approaches. One of them, ProteinMPNN, predicts an amino …

Flavins such as flavin mononucleotide or flavin adenine dinucleotide are bound by diverse
proteins, yet have very similar spectra when in the oxidized state. Recently, we developed …

Flavin‐based fluorescent proteins are oxygen‐independent reporters that hold great
promise for imaging anaerobic and hypoxic biological systems. In this study, we explored …

Directed evolution is a powerful method in biological engineering. Current approaches draw
on time-invariant selection mechanisms, ideal for evolving steady-state properties such as …