Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays

N Fierer, JA Jackson, R Vilgalys… - Applied and …, 2005 - Am Soc Microbiol
Here we describe a quantitative PCR-based approach to estimating the relative abundances
of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for …

Laboratory techniques used for entomopathogenic fungi: Hypocreales

GD Inglis, J Enkerli, MS Goettel - Manual of techniques in …, 2012 - books.google.com
Recent taxonomic studies on fungi using molecular techniques have provided important
information on their phylogenies and new classifications (Chapter VI). Many genera of …

Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis

DK Manter, JM Vivanco - Journal of microbiological methods, 2007 - Elsevier
Molecular-based approaches to assess microbial biomass and diversity from soil and other
ecosystems are rapidly becoming the standard methodology for analysis. While these …

Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR

N Chemidlin Prévost-Bouré, R Christen, S Dequiedt… - PloS one, 2011 - journals.plos.org
Fungi constitute an important group in soil biological diversity and functioning. However,
characterization and knowledge of fungal communities is hampered because few primer …

Soil and leaf litter metaproteomics—a brief guideline from sampling to understanding

KM Keiblinger, S Fuchs… - FEMS microbiology …, 2016 - academic.oup.com
The increasing application of soil metaproteomics is providing unprecedented, in-depth
characterization of the composition and functionality of in situ microbial communities …

Accuracy, precision, and method detection limits of quantitative PCR for airborne bacteria and fungi

D Hospodsky, N Yamamoto… - Applied and environmental …, 2010 - Am Soc Microbiol
Real-time quantitative PCR (qPCR) for rapid and specific enumeration of microbial agents is
finding increased use in aerosol science. The goal of this study was to determine qPCR …

Microbial community shifts influence patterns in tropical forest nitrogen fixation

SC Reed, AR Townsend, CC Cleveland, DR Nemergut - Oecologia, 2010 - Springer
The role of biodiversity in ecosystem function receives substantial attention, yet despite the
diversity and functional relevance of microorganisms, relationships between microbial …

High fungal diversity and abundance recovered in the deep-sea sediments of the Pacific Ocean

W Xu, KL Pang, ZH Luo - Microbial ecology, 2014 - Springer
Abstract Knowledge about the presence and ecological significance of bacteria and archaea
in the deep-sea environments has been well recognized, but the eukaryotic microorganisms …

[HTML][HTML] Rapid and effective DNA extraction method with bead Grindingfor a large amount of fungal DNA

M Watanabe, K Lee, K Goto, S Kumagai… - Journal of food …, 2010 - Elsevier
To identify a rapid method for extracting a large amount of DNA from fungi associated with
food hygiene, extraction methods were compared using fungal pellets formed rapidly in …

Molecular ecology of fungal entomopathogens: molecular genetic tools and their applications in population and fate studies

J Enkerli, F Widmer - BioControl, 2010 - Springer
The power of molecular genetic techniques to address ecological research questions has
opened a distinct interdisciplinary research area collectively referred to as molecular …