Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection

AP Demchenko - Methods and applications in fluorescence, 2020 - iopscience.iop.org
Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy

P Klán, T Solomek, CG Bochet, A Blanc… - Chemical …, 2013 - ACS Publications
Photoremovable Protecting Groups in Chemistry and Biology: Reaction Mechanisms and
Efficacy | Chemical Reviews ACS ACS Publications C&EN CAS Find my institution Log In …

[HTML][HTML] Advanced fluorescence microscopy techniques—Frap, Flip, Flap, Fret and flim

HC Ishikawa-Ankerhold, R Ankerhold, GPC Drummen - Molecules, 2012 - mdpi.com
Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

FRET imaging

EA Jares-Erijman, TM Jovin - Nature biotechnology, 2003 - nature.com
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating
fluorescence signals sensitive to molecular conformation, association, and separation in the …

Visualizing transcription factor dynamics in living cells

Z Liu, R Tjian - Journal of Cell Biology, 2018 - rupress.org
The assembly of sequence-specific enhancer-binding transcription factors (TFs) at cis-
regulatory elements in the genome has long been regarded as the fundamental mechanism …

Labeling of fusion proteins with synthetic fluorophores in live cells

A Keppler, H Pick, C Arrivoli… - Proceedings of the …, 2004 - National Acad Sciences
A general approach for the sequential labeling of fusion proteins of O6-alkylguanine-DNA
alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT …

Forces for morphogenesis investigated with laser microsurgery and quantitative modeling

MS Hutson, Y Tokutake, MS Chang, JW Bloor… - Science, 2003 - science.org
We investigated the forces that connect the genetic program of development to
morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for …

[图书][B] The fungal community: its organization and role in the ecosystem

J Dighton, JF White - 2017 - taylorfrancis.com
"… a number of chapters provide excellent summaries of the modern methods available for
studying fungal ecology, along with those more traditional methods that are still extremely …

Photoswitchable cyan fluorescent protein for protein tracking

DM Chudakov, VV Verkhusha, DB Staroverov… - Nature …, 2004 - nature.com
In recent years diverse photolabeling techniques using green fluorescent protein (GFP)-like
proteins have been reported,,,,,,, including photoactivatable PA-GFP, photoactivatable …

Kindling fluorescent proteins for precise in vivo photolabeling

DM Chudakov, VV Belousov, AG Zaraisky… - Nature …, 2003 - nature.com
Photobleaching of green fluorescent protein (GFP) is a widely used approach for tracking
the movement of subcellular structures and intracellular proteins,,. Although photobleaching …