Application of microfluidic technology in antibody screening

H Sun, N Hu, J Wang - Biotechnology Journal, 2022 - Wiley Online Library
Specific antibodies are widely used in the biomedical field. Current screening methods for
specific antibodies mainly involve hybridoma technology and antibody engineering …

Applications of flow cytometry sorting in the pharmaceutical industry: A review

M Vitelli, H Budman, M Pritzker… - Biotechnology …, 2021 - Wiley Online Library
The article reviews applications of flow cytometry sorting in manufacturing of
pharmaceuticals. Flow cytometry sorting is an extremely powerful tool for monitoring …

Molecularly imprinted polymer nanoparticle-based assay (MINA): application for fumonisin B1 determination

H Munawar, K Smolinska-Kempisty, AG Cruz… - Analyst, 2018 - pubs.rsc.org
The enzyme-linked immunosorbent assay (ELISA) has been used as a standard tool for
monitoring food and animal feed contamination from the carcinogenic fumonisin B1 (FB1) …

Monoclonal antibody discovery based on precise selection of single transgenic hybridomas with an on-cell-surface and antigen-specific anchor

Y Li, P Li, Y Ke, X Yu, W Yu, K Wen… - ACS Applied Materials …, 2022 - ACS Publications
Hybridoma technology is widely used for monoclonal antibody (mAb) discovery, whereas
the generation and identification of single hybridomas by the limiting dilution method (LDM) …

A Dynamic and Pseudo-Homogeneous MBs-icELISA for the Early Detection of Aflatoxin B1 in Food and Feed

L Wei, D Xu, B Yuan, C Pang, H Xu, K Nie, Q Yang… - Toxins, 2023 - mdpi.com
Aflatoxin B1 (AFB1) is one of the most toxic and harmful fungal toxins to humans and
animals, and the fundamental way to prevent its entry into humans is to detect its presence …

Highly efficient and precise two-step cell selection method for tetramethylenedisulfotetramine-specific monoclonal antibody production

Y Li, L Yang, W Yu, X Yu, K Wen, B Shao, J Sun… - Journal of Hazardous …, 2022 - Elsevier
Monoclonal antibodies (mAbs) are useful biological tools for research, diagnostics, and
pharmaceuticals. Here, we proposed a new mAb discovery platform named the two-step cell …

Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins

B Gardill, J Huang, L Tu, F Van Petegem, K Oxenoid… - Scientific Reports, 2020 - nature.com
Multi-pass membrane proteins are important targets of biologic medicines. Given the
inherent difficulties in working with membrane proteins, we sought to investigate the utility of …

Study of epitope imprinting for small templates: Preparation of nanoMIPs for Ochratoxin A

D López‐Puertollano, T Cowen, Á García‐Cruz… - …, 2019 - Wiley Online Library
Ochratoxin A (OTA) is a carcinogenic mycotoxin produced by Aspergillus and Penicillium,
which contaminates cereals like corn and wheat, in addition to beverages such as coffee …

Advances in precise cell manipulation

Y Ma, Z Liang, Y Chen, J Wang - Droplet, 2025 - Wiley Online Library
Research on cells and organ‐like tissues is critical in the fields of molecular biology, genetic
analysis, proteomics analysis, tissue engineering, and others. In recent years …

A microfluidic chip for screening high-producing hybridomas at single cell level

W Zhang, R Li, F Jia, Z Hu, Q Li, Z Wei - Lab on a Chip, 2020 - pubs.rsc.org
Hybridomas are a commonly used, or even the only option, for laboratory study and pilot
production of monoclonal antibodies (mAbs), which are crucial for both targeted therapy and …